Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)

Antibody Source

Polyclonal Sheep IgG
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Product Specifications

Immunogen

E. coli-derived recombinant human Glis1
Met446-Thr620
Accession # Q5VTL4

Specificity

Detects human Glis1 in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant mouse Glis1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human Glis1 Alexa Fluor® 700‑conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Glis1

Glis1 (GLI/glioma-Similar 1; also GliH1) is a 66 kDa (predicted) member of the Kruppel-like zinc finger family of proteins. In the adult, it is principally expressed in placenta and kidney, while in the embryo, it has widespread expression in mesodermal structures. Glis1 can be induced in suprabasal keratinocytes by activating cytokines, and is believed to promote a unique differentiation program. Glis1 is also effective in generating iPSCs from somatic cells (fibroblasts) when concurrently expressed with Oct3, Sox2 and Klf4. Multiple reprogramming pathways are involved, and include Myc, Wnt and Nanog molecules. Functionally, Glis1 is believed to act as a transcriptional repressor.  Although it has been described as possessing an activation domain, it would appear that gene promotion is a consequence of the repression of a "repressor". The consequence sequence ACCACCCA corresponds to a GLIS binding motif. Human Glis1 is 620 amino acids (aa) in length. Based on mouse, it contains a zinc-finger repressor region (aa 197-346) plus a putative transactivation domain (aa 448-620). And again based on mouse, there might be an approximately 796 aa splice form that shows an alternative start site 171 aa upstream of the 620 aa isoform.  The existence of such an isoform would generate an approximately 95-100 kDa isoform in SDS-PAGE. Over aa 446-620, human Glis1 shares 80% aa sequence identity with mouse Glis1.

Long Name

GLIS Family Zinc Finger 1

Alternate Names

FLJ36155, GLIS family zinc finger 1, GLI-similar 1, zinc finger protein GLIS1

Entrez Gene IDs

148979 (Human); 230587 (Mouse); 298732 (Rat)

Gene Symbol

GLIS1

UniProt

Additional Glis1 Products

Product Documents for Human Glis1 Alexa Fluor® 700‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Glis1 Alexa Fluor® 700‑conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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