Human Glis1 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Glis1 in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant mouse Glis1 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Glis1
    Met446-Thr620
    Accession # Q5VTL4
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Glis1 by Western Blot. Western blot shows lysates of HEK001 human epidermal keratinocyte cell line, WS‑1 human fetal skin fibroblast cell line, U‑118‑MG human glioblastoma/astrocytoma cell line, and U‑87 MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human Glis1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7618) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Glis1 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Glis1

Glis1 (GLI/glioma-Similar 1; also GliH1) is a 66 kDa (predicted) member of the Kruppel-like zinc finger family of proteins. In the adult, it is principally expressed in placenta and kidney, while in the embryo, it has widespread expression in mesodermal structures. Glis1 can be induced in suprabasal keratinocytes by activating cytokines, and is believed to promote a unique differentiation program. Glis1 is also effective in generating iPSCs from somatic cells (fibroblasts) when concurrently expressed with Oct3, Sox2 and Klf4. Multiple reprogramming pathways are involved, and include Myc, Wnt and Nanog molecules. Functionally, Glis1 is believed to act as a transcriptional repressor.  Although it has been described as possessing an activation domain, it would appear that gene promotion is a consequence of the repression of a "repressor". The consequence sequence ACCACCCA corresponds to a GLIS binding motif. Human Glis1 is 620 amino acids (aa) in length. Based on mouse, it contains a zinc-finger repressor region (aa 197-346) plus a putative transactivation domain (aa 448-620). And again based on mouse, there might be an approximately 796 aa splice form that shows an alternative start site 171 aa upstream of the 620 aa isoform.  The existence of such an isoform would generate an approximately 95-100 kDa isoform in SDS-PAGE. Over aa 446-620, human Glis1 shares 80% aa sequence identity with mouse Glis1.

  • Long Name:
    GLIS Family Zinc Finger 1
  • Entrez Gene IDs:
    148979 (Human); 230587 (Mouse); 298732 (Rat)
  • Alternate Names:
    FLJ36155; GLIS family zinc finger 1; Glis1; GLI-similar 1; zinc finger protein GLIS1
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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