Human GPR15 Alexa Fluor® 405-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB3654V-100UG
Detection of GPR15 in HEK293 Human Cell Line Transfected with Human GPR15 and eGFP by Flow Cytometry.
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Human GPR15 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Stains human GPR15-transfected cells but not irrelevant transfectants.
Source
Monoclonal Mouse IgG2B Clone # 367902
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human GPR15
Met1-Leu360
Accession # P49685
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 405

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of GPR15 in HEK293 Human Cell Line Transfected with Human GPR15 and eGFP by Flow Cytometry. View Larger

Detection of GPR15 in HEK293 Human Cell Line Transfected with Human GPR15 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human GPR15 and eGFP was stained with either (A) Mouse Anti-Human GPR15 Alexa Fluor® 405‑conjugated Monoclonal Antibody (Catalog # FAB3654V) or (B) Mouse IgG2B Alexa Fluor® 405 Isotype Control (Catalog # IC0041V). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: GPR15

GPR15, also known as BOB, is a seven-transmembrane G protein-coupled receptor that is expressed in CD4+ T cells and alveolar macrophages. GPR15 functions as a cellular co-receptor for some isolates of HIV-1, HIV-2, and SIV through interactions with several viral envelope proteins. Human GPR15 shares 96%‑100% amino acid sequence identity with chimpanzee, macaque, and rhesus GPR15, and 76% with mouse GPR15.

Long Name
G Protein-coupled Receptor 15
Entrez Gene IDs
2838 (Human); 71223 (Mouse)
Alternate Names
Bob; Brother of Bonzo; G protein-coupled receptor 15; GPR15; G-protein coupled receptor 15; MGC126828; MGC126830

Product Datasheets

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