Human GPR15 Alexa Fluor® 405-conjugated Antibody

FAB3654V Datasheet / COA / SDS

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FAB3654V-100UG

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Detection of GPR15 in HEK293 Human Cell Line Transfected with Human GPR15 and eGFP by Flow Cytometry.

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Human GPR15 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Stains human GPR15-transfected cells but not irrelevant transfectants.

Source

Monoclonal Mouse IgG_2B Clone # 367902

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human GPR15
Met1-Leu360
Accession # P49685

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry

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Detection of GPR15 in HEK293 Human Cell Line Transfected with Human GPR15 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human GPR15 and eGFP was stained with either (A) Mouse Anti-Human GPR15 Alexa Fluor® 405-conjugated Monoclonal Antibody (Catalog # FAB3654V) or (B) Mouse IgG2B Alexa Fluor® 405 Isotype Control (Catalog # IC0041V). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: GPR15

GPR15, also known as BOB, is a seven-transmembrane G protein-coupled receptor that is expressed in CD4⁺ T cells and alveolar macrophages. GPR15 functions as a cellular co-receptor for some isolates of HIV-1, HIV-2, and SIV through interactions with several viral envelope proteins. Human GPR15 shares 96%‑100% amino acid sequence identity with chimpanzee, macaque, and rhesus GPR15, and 76% with mouse GPR15.

Long Name

G Protein-coupled Receptor 15

Entrez Gene IDs

2838 (Human); 71223 (Mouse)

Alternate Names

Bob; Brother of Bonzo; G protein-coupled receptor 15; GPR15; G-protein coupled receptor 15; MGC126828; MGC126830