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Human Histone H2AX Alexa Fluor® 405-conjugated Antibody

Catalog #: FAB11529V Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB11529V-100UG

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Human Histone H2AX Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Histone H2AX.
Source
Monoclonal Mouse IgG2b Clone # 1077751
Purification
Protein A or G purified
Immunogen
E. coli-derived recombinant human H2AX
Gly3-Ser131
Accession # P16104
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration
Sample
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Histone H2AX

Histone H2AX is a core histone protein that is phosphorylated at S139 in cells exposed to DNA double-strand break-inducing agents, such as ionizing radiation. The S139 phosphorylated H2AX, termed gamma -H2AX, marks the site of DNA double-strand breaks and serves to recruit cell cycle checkpoint and DNA repair factors to the site of damage.

Entrez Gene IDs
3014 (Human)
Alternate Names
H2A.X; H2A/X; H2AFX; Histone H2AX

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