Home / Histone H2AX / Human Histone H2AX Alexa Fluor® 488-conjugated Antibody

Human Histone H2AX Alexa Fluor® 488-conjugated Antibody

IC2288G Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
IC2288G-100UG
Product Details
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Histone H2AX Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Histone H2AX when phosphorylated at S139 in Western blots.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2207D
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Phospho-peptide containing human Histone H2AX S139 site
Accession # P16104
Formulation
PBS.Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
Camptothecin-treated Human PBMC, fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with 90% methanol

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Histone H2AX

Histone H2AX is a core histone protein that is phosphorylated at S139 in cells exposed to DNA double-strand break-inducing agents, such as ionizing radiation. The S139 phosphorylated H2AX, termed gamma -H2AX, marks the site of DNA double-strand breaks and serves to recruit cell cycle checkpoint and DNA repair factors to the site of damage.

Entrez Gene IDs
3014 (Human)
Alternate Names
H2A.X; H2A/X; H2AFX; Histone H2AX

Product Datasheets

Product Datasheet
COA
SDS

FAQs

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Isotype Controls

Rabbit IgG Alexa Fluor® 488-conjugated Antibody

IC1051G
Ctrl

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