Human Histone H2AX Alexa Fluor® 750-conjugated Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
IC2288S-100UG
Product Details
FAQs
Supplemental Products
Reviews

Human Histone H2AX Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Histone H2AX when phosphorylated at S139 in Western blots.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2207D
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Phospho-peptide containing human Histone H2AX S139 site
Accession # P16104
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Complete Your Research

Complete Your Experiment

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
Camptothecin-treated Human PBMC, fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with 90% methanol

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Histone H2AX

Histone H2AX is a core histone protein that is phosphorylated at S139 in cells exposed to DNA double-strand break-inducing agents, such as ionizing radiation. The S139 phosphorylated H2AX, termed gamma -H2AX, marks the site of DNA double-strand breaks and serves to recruit cell cycle checkpoint and DNA repair factors to the site of damage.

Entrez Gene IDs
3014 (Human)
Alternate Names
H2A.X; H2A/X; H2AFX; Histone H2AX

Product Datasheets

You must select a language.

x

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reviews for Human Histone H2AX Alexa Fluor® 750-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human Histone H2AX Alexa Fluor® 750-conjugated Antibody and earn rewards!

Have you used Human Histone H2AX Alexa Fluor® 750-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review