Human Phospho-Histone H2AX (S139) Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB2288-100
MAB2288-SP
Detection of Human Histone H2AX by Western Blot.
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Human Phospho-Histone H2AX (S139) Antibody Summary

Species Reactivity
Human
Specificity
Detects human Histone H2AX when phosphorylated at S139 in Western blots.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2207D
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Phospho-peptide containing human Histone H2AX S139 site
Accession # P16104
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Histone H2AX antibody by Western Blot. View Larger

Detection of Human Histone H2AX by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 20 mJ/cm2 ultraviolet light (UV) followed by a 30 minute recover. PVDF membrane was probed with 0.25 µg/mL of Rabbit Anti-Human Phospho-Histone H2AX (S139) Monoclonal Antibody (Catalog # MAB2288) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Histone H2AX at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Intracellular Staining by Flow Cytometry Detection of Human Phospho-Histone H2AX (S139) antibody in Camptothecin-treated PBMC antibody by Flow Cytometry. View Larger

Detection of Human Phospho-Histone H2AX (S139) in Camptothecin-treated PBMC by Flow Cytometry. Human PBMC untreated (open histogram) or treated with 1 µM Camptothecin (Catalog # 1100, filled histogram) overnight were stained with Rabbit Anti-Human Phospho-Histone H2AX (S139) Monoclonal Antibody (Catalog # MAB2288), followed by PE-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with 90% methanol. View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Histone H2AX

Histone H2AX is a core histone protein that is phosphorylated at S139 in cells exposed to DNA double-strand break-inducing agents, such as ionizing radiation. The S139 phosphorylated H2AX, termed gamma -H2AX, marks the site of DNA double-strand breaks and serves to recruit cell cycle checkpoint and DNA repair factors to the site of damage.

Entrez Gene IDs
3014 (Human)
Alternate Names
H2A.X; H2A/X; H2AFX; Histone H2AX

Product Datasheets

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Human Phospho-Histone H2AX (S139) Antibody
By Anonymous on 07/16/2018
Application: WB Sample Tested: PC-3 human prostate cancer cell line Species: Human

PC3 cells were treated with UV light for 1h. Total cell lysates were subjected to western blot. PVDF membrane were probed with 1mm/ml Human Phospho-Histone H2AX (S139) (MAB2288). A specific band was detected for p-H2AX at approximately 17 kDa. This experiment was conducted under reducing conditions