Human IL-20 R alpha APC-conjugated Antibody

FAB11762A has been discontinued. View all IL-20 R alpha products.
  
  • Species Reactivity
    Human
  • Specificity
    Detects human IL-20 R alpha in direct ELISAs and Western blots. In Western blots, approximately 5‑15% cross‑reactivity with recombinant human (rh) IFN-gamma R2, rhIL-10 R, rhIL-10 R beta, rhIL-20 R beta, and rhIL-22 BP is observed and no cross-reactivity with rhIFN-gamma RI, recombinant mouse IL‑20 R alpha, or rhIL‑22 R is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 173714
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human IL-20 R alpha
    Val30-Lys250
    Accession # Q9UHF4
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of IL‑20 R alpha in BaF3 Mouse Cell Line Transfected with Human IL-20 R alpha  by Flow Cytometry. BaF3 mouse pro-B cell line transfected with Human IL-20 R alpha and eGFP was stained with Mouse Anti-Human IL‑20 R alpha APC‑conjugated Monoclonal Antibody (Catalog # FAB11762A). Quadrant markers were set based on control antibody staining. View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-20 R alpha

IL-20 receptor alpha (IL-20 R alpha ), also named IL-20 R1, CRF2-8, and ZCYTOR7, belongs to the class II cytokine receptor family, which includes 12 members. These receptors are characterized by the patterns of conserved amino acid (aa) residues in their extracellular domains, which are composed of tandem fibronectin type III domains (1). Class II cytokine receptors form heterodimeric signaling receptor complexes that mediate class II cytokine signals. Subunits of the different receptor complexes are shared and serve multiple functions (1).

The gene for human IL-20 R alpha is mapped to chromosome 6 and encodes a 553 aa glycoprotein with a 29 aa signal peptide, a 221 aa extracellular domain, a 24 aa transmembrane region and a 279 aa intracellular domain (2). IL-20 R alpha is widely expressed and is detected at high levels in multiple tissues including skin, testis, heart, placenta, salivary gland and prostate gland (1). The expression of IL-20 R alpha, together with that of IL-20 R beta, is upregulated in psoriatic skin lesions on keratinocytes, immune cells, and endothelial cells (1, 2).

IL-20 R alpha heterodimerizes with IL-20 R beta to form the functional receptor that mediates IL-19, IL-20 and IL-24 signals (3, 4). IL-20 R alpha also heterodimerizes with IL-10 R beta to form the functional receptor complex for IL-26 (5). Binding of these IL-10 family class II cytokines to their functional receptors induces activation of the JAK-STAT signal transduction pathway. At low ligand concentrations, STAT3 has been shown to be the predominant STAT proteins activated through either complexes (3‑5).

  • References:
    1. Kotenko, S.V. (2003) Cytokine & Growth Factor Reviews 13:223.
    2. Xie, M.H. et al. (2000) J. Biol. Chem. 275:31335.
    3. Dumoutier, L. et al. (2001) J. Immunol. 167:3534.
    4. Parrish-Novak, J. et al. (2002) J. Biol. Chem. 277:47517s.
    5. Sheikh. F. et al. (2004) J. Immunol. 172:2006.
  • Long Name:
    Interleukin 20 Receptor alpha
  • Entrez Gene IDs:
    53832 (Human); 237313 (Mouse)
  • Alternate Names:
    class II cytokine receptor ZCYTOR7; CRF2-8; Cytokine receptor class-II member 8; Cytokine receptor family 2 member 8; FLJ40993; IL-20 R alpha; IL20R alpha; IL-20R1IL-20 receptor subunit alpha; IL20RA; IL-20Ra; IL-20R-alpha; interleukin 20 receptor, alpha; interleukin-20 receptor I; interleukin-20 receptor subunit alpha; zcytor7
Related Research Areas

Have you used Human IL-20 R alpha APC-conjugated Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information