Detects human IL-20 R alpha in direct ELISAs and Western blots. In Western blots, approximately 5‑15% cross‑reactivity with recombinant human (rh) IFN-gamma R2, rhIL-10 R, rhIL-10 R beta, rhIL-20 R beta, and rhIL-22 BP is observed and no cross-reactivity with rhIFN-gamma RI, recombinant mouse IL‑20 R alpha, or rhIL‑22 R is observed.
Monoclonal Mouse IgG1 Clone # 173714
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human IL-20 R alpha Val30-Lys250 Accession # Q9UHF4
Supplied in a saline solution containing BSA and Sodium Azide.
Detection of IL‑20 R alpha in BaF3 Mouse Cell Line Transfected with Human IL-20 R alpha by Flow Cytometry.
BaF3 mouse pro-B cell line transfected with Human IL-20 R alpha and eGFP was stained with Mouse Anti-Human IL‑20 R alpha APC‑conjugated Monoclonal Antibody (Catalog # FAB11762A). Quadrant markers were set based on control antibody staining. View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-20 R alpha
IL-20 receptor alpha (IL-20 R alpha ), also named IL-20 R1, CRF2-8, and ZCYTOR7, belongs to the class II cytokine receptor family, which includes 12 members. These receptors are characterized by the patterns of conserved amino acid (aa) residues in their extracellular domains, which are composed of tandem fibronectin type III domains (1). Class II cytokine receptors form heterodimeric signaling receptor complexes that mediate class II cytokine signals. Subunits of the different receptor complexes are shared and serve multiple functions (1).
The gene for human IL-20 R alpha is mapped to chromosome 6 and encodes a 553 aa glycoprotein with a 29 aa signal peptide, a 221 aa extracellular domain, a 24 aa transmembrane region and a 279 aa intracellular domain (2). IL-20 R alpha is widely expressed and is detected at high levels in multiple tissues including skin, testis, heart, placenta, salivary gland and prostate gland (1). The expression of IL-20 R alpha, together with that of IL-20 R beta, is upregulated in psoriatic skin lesions on keratinocytes, immune cells, and endothelial cells (1, 2).
IL-20 R alpha heterodimerizes with IL-20 R beta to form the functional receptor that mediates IL-19, IL-20 and IL-24 signals (3, 4). IL-20 R alpha also heterodimerizes with IL-10 R beta to form the functional receptor complex for IL-26 (5). Binding of these IL-10 family class II cytokines to their functional receptors induces activation of the JAK-STAT signal transduction pathway. At low ligand concentrations, STAT3 has been shown to be the predominant STAT proteins activated through either complexes (3‑5).
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