Human Luminex Assay

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Human Luminex Assay Summary

Assay Type Polystyrene bead-based multiplex assay for the Luminex® platform
Format 1 x 96-well microplate and Polystyrene antibody-coated beads
Species Human
Analytes Detected Please see analyte list in assay customization tool below.
Performance Validation Luminex Assays are validated for use with cell culture supernatants, plasma and serum. All Luminex assays are tested for sensitivity, intra-assay precision, inter-assay precision and to ensure assay linearity for validated sample types. Antibody pairs are selected and tested to confirm the parallel detection of natural and recombinant standard protein and to ensure the accurate determination of target analytes within biological samples. Assays for each target analyte are screened against all target analytes to confirm low antibody cross-reactivity.

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Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Ordering Tool. We also offer Custom Luminex Services

Product Features

Polystyrene Luminex Assays are the most flexible bead-based multiplex assays that we offer. They allow up to 100 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples.

  • Simultaneously profile up to 100 analytes of your choice in one sample
  • Choose from a wide menu of analytes, including many unique analytes
  • Requires a small sample volume (<50 µL)
  • Run your assay in just 3-4 hours
  • Mass-calibrated standards for consistent results with every new lot of material
  • Enough reagents for one 96-well plate
  • Polystyrene kits are typically shipped within 21-28 days of ordering.*

Please allow additional time for international orders.

Kit Components
  • Premixed cocktail of antibody-coated Polystyrene beads
  • Premixed cocktail of biotinylated detection antibody
  • Standard Cocktail(s)
  • Bead Diluent
  • Biotin Antibody Diluent
  • Standard/Sample Diluent
  • Wash Buffer
  • Streptavidin-PE
  • One filter-bottom 96-well Microplate
  • Foil Plate Sealers (4)
  • Mixing Bottle
  • Certificate of Analysis**

**A standard curve must be generated each time an assay is run, utilizing values from the Certificate of Analysis provided with each Luminex Assay.

Which Luminex Assay is right for me?

Assays for the Luminex platform are offered as High Performance Assays or Assays. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays.

Product Datasheets

FAQs

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