Human Magnetic Luminex® Performance Assay MMP Base Kit
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Human Magnetic Luminex® Performance Assay MMP Base Kit Summary
|Panel||Luminex Performance Human MMP Panel|
|Assay Type||Magnetic bead-based multiplex assay for the Luminex® platform|
|Format||1 x 96-well microplate and Magnetic antibody-coated beads|
|Analytes Detected||Please see analyte list in assay customization tool below.|
|Performance Validation||Our Luminex High Performance Assays undergo our most extensive validation testing. All Luminex High Performance Assay Panels are validated for use with serum and plasma samples. Some High Performance Assays are additionally validated for cell culture supernatants, milk, saliva, or urine, as indicated on the individual product data sheets. Luminex High Performance Assays are tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types. Recovery values for individual samples in validated sample types are also tested. Validation data for each analyte can be found in the product datasheet.
Make your selections below to configure your assay
Below are kits that are available based on your selections. These selections may be further customized before adding to the cart or emailing the configuration to your purchasing department or local distributor to place your order. Selected analytes may be included in more than one High Performance Assay. All assay options are displayed.
If you run into bead region incompatibilities, send your Luminex code to our team and find out more.
How would you like these analytes mixed?
Would you like to split based on dilution?
Magnetic Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples.
- Simultaneously profile up to analytes of your choice in one sample
- Magnetic format allows for easier wash steps
- Undergoes similar validation testing as Quantikine® ELISA Kits
- Precise and Specific
- Requires a small sample volume (<50 µL)
- Run your assay in just 3-5 hours
- Mass-calibrated standards for consistent results with every new lot of material
- Enough reagents for one 96-well plate
- Standard Cocktail(s)
- Bead Diluent
- Biotin Antibody Diluent
- Calibrator Diluent
- Wash Buffer
- One flat-bottom 96-well Microplate
- Foil Plate Sealers (4)
- Mixing Bottle
- Standard Value Card**
**A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Base Kit.
- User-mix antibody-coated Magnetic beads in individual vials
- User-mixed cocktail of biotinylated detection antibody
Assays for the Luminex platform are offered as High Performance Assays or Assays. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays.
Magnetic Luminex High Performance Assays are designed for use with the Luminex MAGPIX CCD Imager. Alternatively, kits can be used with the Luminex 100/200™ or FLEXMAP 3D, dual laser, flow-based sorting and detection platforms.
Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. One laser is bead-specific and determines which analyte is being detected. A magnet in the analyzer captures and holds the superparaMagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a Bio-Rad Bio-Plex dual laser, flow-based systems.
Citations for Human Magnetic Luminex® Performance Assay MMP Base Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 10
Filter your results:
Increased Matrix Metalloproteinase-2 and Matrix Metalloproteinase-3 Concentrations in Corneal Epithelium of Patients with Recurrent Corneal Erosions
Authors: K Jadczyk-So, W Garczorz, B Buba?a-Sta, T Francuz, E Mrukwa-Kom
Journal of Ophthalmology, 2022;2022(0):5024037.
Sample Types: Cell Lysates
Effect of BCG vaccination on proinflammatory responses in elderly individuals
Authors: N Pavan Kuma, C Padmapriya, A Rajamanick, SB Marinaik, A Nancy, S Padmanaban, N Akbar, M Murhekar, S Babu
Science Advances, 2021;7(32):.
Sample Types: Plasma
Evaluation of serum MMP-2 and MMP-3, synovial fluid IL-8, MCP-1, and KC concentrations as biomarkers of stifle osteoarthritis associated with naturally occurring cranial cruciate ligament rupture in dogs
Authors: S Malek, HY Weng, SA Martinson, MC Rochat, R Béraud, CB Riley
PLoS ONE, 2020;15(11):e0242614.
Sample Types: Serum
Plasma levels of C-reactive protein, matrix metalloproteinase-7 and lipopolysaccharide-binding protein distinguish active pulmonary or extrapulmonary tuberculosis from uninfected controls in children
Authors: VVS Albuquerqu, NP Kumar, KF Fukutani, B Vasconcelo, MB Arriaga, PS Silveira-M, S Babu, BB Andrade
Sample Types: Plasma
Effects of aqueous suppressants and prostaglandin analogues on early wound healing after glaucoma implant surgery
Authors: KI Jung, JE Woo, CK Park
Sci Rep, 2019;9(1):5251.
Sample Types: Aqueous Humor
Characterization of the local wound environment following treatment of chronic leg ulcers with SIS wound matrix
Authors: JP Hodde, MC Hiles, DW Metzger
J Tissue Viability, 2019;0(0):.
Sample Types: Wound Fluid
Marked infiltration of neutrophils at the upper palpebral conjunctiva in patients with chronic graft-versus-host disease
Authors: YS Byun, YS Yoo, MJ Kang, WJ Whang, KS Na, JW Mok, CK Joo
Ocul Surf, 2018;0(0):.
Glucocorticoid sensitivity and inflammatory status of peripheral blood mononuclear cells in patients with coronary artery disease
Authors: S Jönsson, AK Lundberg, RWS Chung, L Jonasson
Ann. Med., 2018;0(0):1-24.
Sample Types: Plasma
The impact of human adipose tissue-derived stem cells on breast cancer cells: implications for cell-assisted lipotransfers in breast reconstruction
Authors: E Koellenspe, LC Bonnert, I Zoernig, F Marmé, S Sandmann, G Germann, F Gramley, U Leimer
Stem Cell Res Ther, 2017;8(1):121.
Sample Types: Cell Culture Supernates
Tumor Necrosis Factor-alpha, Matrix-Metalloproteinases 8 and 9 Levels in the Saliva Are Associated with Increased Hemoglobin A1c in Type 1 Diabetes Subjects.
Authors: Kuehl M, Rodriguez H, Burkhardt B, Alman A
PLoS ONE, 2015;10(4):e0125320.
Sample Types: Saliva
Overexpression of MMP-9 and its inhibitors in blood mononuclear cells after myocardial infarction--is it associated with depressive symptomatology?
Authors: Jonsson S, Lundberg A, Jonasson L
PLoS ONE, 2014;9(8):e105572.
Sample Types: Cell Culture Supernates
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