Human Olig3 Affinity Purified PAb

Catalog #: AF2356 Datasheet / COA / SDS

Discontinued Product

AF2356 has been discontinued.
View all Olig3 products.
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Human Olig3 Affinity Purified PAb Summary

Species Reactivity
Human
Specificity
Detects human Olig3 in direct ELISAs and Western blots. In Western blots, less than 2% cross-reactivity with recombinant human (rh) Olig-1 and rhOlig-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Olig3
Met1-Lys272
Accession # Q7RTU3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Olig3
Immunohistochemistry
5-15 µg/mL
Immersion fixed frozen sections of embryonic mouse spinal cord (E11)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Olig3

The Olig family is a sub-family of the basic helix-loop-helix transcription factors. Olig1 and Olig2, which have been implicated in oligodendrogenesis, are expressed in the region of the ventral ventricular zone of late embryonic spinal cord where oligodendrocyte progenitors appear. Olig3 is transiently expressed in different types of progenitors of embryonic central nervous system and then disappears in the course of development. In adult, expression of Olig3 is primarily detected in non-neural tissues.

Long Name
Oligodendrocyte Lineage Transcription Factor 3
Entrez Gene IDs
167826 (Human); 94222 (Mouse)
Alternate Names
BHLHB7; BHLHE20; bHLHe20oligo3; Class B basic helix-loop-helix protein 7; Class E basic helix-loop-helix protein 20; Olig3; Oligo3; oligodendrocyte transcription factor 3

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