Human PD‑1 (Research Grade Pembrolizumab Biosimilar) Antibody

R&D Systems | Catalog # MAB11380

Recombinant Monoclonal Antibody.
R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG4 kappa Clone # Hu121
View all PD-1 Primary Antibodies »

Product Specifications

Immunogen

Human PD-1

Specificity

Detects human PD-1 in direct ELISA. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Pembrolizumab. This product is for research use only.

Clonality

Monoclonal

Host

Human

Isotype

IgG4 kappa

Scientific Data Images for Human PD‑1 (Research Grade Pembrolizumab Biosimilar) Antibody

Detection of PD‑1 in HEK 293 cells by Flow Cytometry.

HEK 293 cells transfected with eGFP and hPD-1 (A) or irrelevant protein (B) were stained with Human Anti-Human PD-1 (Research Grade PembrolizumabBiosimilar) Monoclonal Antibody (Catalog # MAB11380) followed by Goat Anti-Human IgG Allophycocyanin (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins.

Applications for Human PD‑1 (Research Grade Pembrolizumab Biosimilar) Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: HEK 293 cells transfected with eGFP and hPD-1 or irrelevant protein

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Advanced Features

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  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PD-1

Pembrolizumab is a humanized monoclonal IgG4 kappa PD-1 blocking antibody.  PD-1 (programmed death receptor-1) is expressed on the surface of lymphocytes, and is also considered an immune checkpoint. The binding of the antibody with PD-1 interferes with the interaction with its ligands PD-L1 and PD-L2 preventing the inhibition of TCR-mediated T-cell proliferation.  The inhibition of this PD-1 pathway results in enhanced immunosurveillance and anti-tumor immune response.  The original therapeutic has been approved by the FDA for refractory melanoma, as well as other types of cancers such as non-small cell lung cancer, cervical cancer, head and neck cancer and Hodgkin's lymphoma.

Long Name

Programmed Death-1

Alternate Names

CD279, PD1, PDCD1, SLEB2

Entrez Gene IDs

5133 (Human); 18566 (Mouse); 301626 (Rat); 100533201 (Porcine); 486213 (Canine); 102123659 (Cynomolgus Monkey)

Gene Symbol

PDCD1

Additional PD-1 Products

Product Documents for Human PD‑1 (Research Grade Pembrolizumab Biosimilar) Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PD‑1 (Research Grade Pembrolizumab Biosimilar) Antibody

For research use only

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