Human IgG APC‑conjugated Antibody

R&D Systems | Catalog # F0135

R&D Systems
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Polyclonal Goat IgG
View all IgG Secondary Antibodies »

Product Specifications

Immunogen

Human IgG

Specificity

This antibody reacts with heavy chains on human IgG and with light chains on all human immunoglobulins. No reactivity is observed to non-immunoglobulin human, mouse, rabbit or bovine serum proteins by IEP.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human IgG APC‑conjugated Antibody

Detection of EGF R/ErbB1 in A431 human epithelial carcinoma cell line by Flow Cytometry.

Detection of EGF R/ErbB1 in A431 human epithelial carcinoma cell line by Flow Cytometry.

A431 human epithelial carcinoma cell line was stained with Human Anti-Human EGF R/ErbB1 (Cetuximab) Monoclonal Antibody (Catalog # MAB9577, filled histogram) or irrelevant antibody (open histogram) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog #F0135). View our protocol for Staining Membrane-associated Proteins.

Applications for Human IgG APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of human origin, and is of IgG class. The recommended concentration is 10 μL/106 cells.

Reviewed Applications

Read 1 review rated 5 using F0135 in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Products

Product Documents for Human IgG APC‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IgG APC‑conjugated Antibody

For research use only

Citations for Human IgG APC‑conjugated Antibody

Customer Reviews for Human IgG APC‑conjugated Antibody (1)

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Customer Images

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  • Human IgG APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Peripheral blood mononuclear cells (PBMCs)
    Species: Canine
    Verified Customer | Posted 06/26/2020
    Human IgG APC‑conjugated Antibody F0135

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