Phospho-STAT2 (Y690) Alexa Fluor® 405‑conjugated Antibody

R&D Systems | Catalog # IC2890V

Recombinant Monoclonal Antibody.
R&D Systems
Discontinued Product
IC2890V has been discontinued. View all STAT2 products.

Key Product Details

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry

Label

Alexa Fluor 405 (Excitation = 405 nm, Emission = 421 nm)

Antibody Source

Monoclonal Rabbit IgG Clone # 1021D
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Product Specifications

Immunogen

Phosphopeptide containing the human STAT2 Y690 site

Specificity

Detects human phospho-STAT2 (Y690) in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Applications for Phospho-STAT2 (Y690) Alexa Fluor® 405‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

0.25-1 µg/106 cells
Sample: Daudi human Burkitt's lymphoma cell line treated with Recombinant Human IFN‑ alpha A (Catalog # 11100-1) was fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with 90% methanol

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months, 2 to 8 °C under sterile conditions after opening.
  • Background: STAT2

    STAT2 (signal transducer and activator of transcription 2) is a 113 kDa member of the STAT family of cytoplasmic transcription factors. STAT members generally mediate cytokine, growth factor and hormone receptor signal transduction. STAT2 is associated with type I ( alpha - and beta -) interferon signaling. All STATs contain an N‑terminal oligomerization domain, a DNA-binding domain, and an SH2-association region. STAT2 is phosphorylated at Y690 by receptor-associated Janus kinases (JAKs) leading to STAT2 dimerization and subsequent translocation to the nucleus to activate gene transcription.

    Long Name

    Signal Transducer and Activator of Transcription 2

    Alternate Names

    interferon alpha induced transcriptional activator, ISGF-3, MGC59816, P113, signal transducer and activator of transcription 2, signal transducer and activator of transcription 2, 113kD, signal transducer and activator of transcription 2, 113kDa, STAT113

    Entrez Gene IDs

    6773 (Human); 20847 (Mouse)

    Gene Symbol

    STAT2

    Additional STAT2 Products

    Product Documents for Phospho-STAT2 (Y690) Alexa Fluor® 405‑conjugated Antibody

    Certificate of Analysis

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    Note: Certificate of Analysis not available for kit components.

    Product Specific Notices for Phospho-STAT2 (Y690) Alexa Fluor® 405‑conjugated Antibody


    This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

    For research use only

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    Associated Pathways

    Jak/STAT Signaling Pathway Jak/STAT Signaling Pathway Thumbnail