Human PSG1 Quantikine ELISA Kit

  (1 citations)     
Datasheet / CoA / SDS
Product Details
Assay Procedure
Citations (1)
FAQs
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 ng/mL), Serum (50 ng/mL), EDTA Plasma (50 ng/mL), Heparin Plasma (50 ng/mL)
  • Sensitivity
    0.48 ng/mL
  • Assay Range
    3.1 - 200 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human PSG-1
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC150 , Quantikine Immunoassay Control Set 744 for Human PSG1 - Please Inquire
Product Summary
The Quantikine Human PSG-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human PSG-1 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human PSG-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human PSG-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human PSG-1.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 18.7 59.4 118 20 62.4 121
Standard Deviation 0.36 0.79 1.7 1.2 2.4 4.9
CV% 1.9 1.3 1.4 6 3.8 4

Recovery

The recovery of PSG-1 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 100 83-118
EDTA Plasma (n=4) 92 84-108
Heparin Plasma (n=4) 90 83-99
Serum (n=4) 91 81-104
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of PSG-1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 PSG1 [HRP]
Product Datasheets

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Note: Certificate of Analysis not available for kit components.

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Note: Certificate of Analysis not available for kit components.

Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: PSG1
Pregnancy-specific beta-1 glycoprotein 1 (PSG-1) is also known as SP1, PSbG1, or B1G1, and is designated CD66f. PSGs are part of the carcinoembryonic antigen (CEA) family and serve as early biochemical markers of syncytiotrophoblast formation. PSGs, which are produced by syncytial cells in the placenta, are detectable in the maternal plasma as early as 2-3 weeks following fertilization and increase as pregnancy progresses. They are alleged to promote immune tolerance of the semi-allotypic fetus during pregnancy. PSG-1 fosters an anti-inflammatory uterine environment by inducing IL-10, IL-6, and TGF-beta 1 secretion. Additionally, PSG-1 is believed to mediate placental vascular morphogenesis by enhancing VEGF-A production and endothelial tube formation.
  • Long Name:
    Pregnancy-specific beta-1-glycoprotein 1
  • Entrez Gene IDs:
    5669 (Human)
  • Alternate Names:
    B1G1; B1G1CD66 antigen-like family member F; CD66f antigen; CD66f; DHFRP2; Fetal liver non-specific cross-reactive antigen 1/2; FLJ90598; FLJ90654; FL-NCA-1/2; PBG1; pregnancy specific beta-1-glycoprotein 1; pregnancy-specific B-1 glycoprotein; Pregnancy-specific beta-1 glycoprotein C/D; pregnancy-specific beta-1-glycoprotein 1; Pregnancy-specific glycoprotein 1; PS-beta-C/D; PS-beta-G-1; PSbG1; PSBG-1; PSBG1SP1; PSG1; PSG-1; PSG95; PSGGA; PSGGAPSG95; PSGIIA
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Pregnancy Specific ?-1 Glycoprotein 1 is Expressed in Pancreatic Ductal Adenocarcinoma and its Subcellular Localization Correlates with Overall Survival
    J Cancer, 2016;7(14):2018-2027.
    Species: Human
    Sample Type: Plasma

FAQs

  1. What is the normal appearance of Assay Diluent RD1-75?

    • Assay Diluent RD1-75 contains high concentrations of additives and typically has a very cloudy, brown appearance. It is recommended to mix this diluent well before and during use.

View all ELISA FAQs
ELISA Controls
Description Application Cat# Citations Images  

Quantikine Immunoassay Control Set 744 for Human PSG1

Ctrl QC150
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Supplemental ELISA Products
Description Application Cat# Citations Images  

Quantikine ELISA Wash Buffer 1

ELISA WA126 6  
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