Pregnancy-specific beta-1 glycoprotein 1 (PSG1) is also known as SP1, PSbG1, or B1G1, and is designated CD66f. PSGs are part of the carcinoembryonic antigen (CEA) family and serve as early biochemical markers of syncytiotrophoblast formation. PSGs, which are produced by syncytial cells in the placenta, are detectable in the maternal plasma as early as 2-3 weeks following fertilization and increase as pregnancy progresses. They are alleged to promote immune tolerance of the semi-allotypic fetus during pregnancy. PSG1 fosters an anti-inflammatory uterine environment by inducing IL-10, IL-6, and TGF-beta 1 secretion. Additionally, PSG1 is believed to mediate placental vascular morphogenesis by enhancing VEGF-A production and endothelial tube formation.
Human PSG1 Quantikine ELISA Kit
R&D Systems | Catalog # DPSG10
Key Product Details
Assay Length
Sample Type & Volume Required Per Well
Sensitivity
Assay Range
Product Summary for Human PSG1 Quantikine ELISA Kit
Product Specifications
Assay Type
Format
Measurement
Detection Method
Conjugate
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 18.7 | 59.4 | 118 | 20.0 | 62.4 | 121 |
| Standard Deviation | 0.36 | 0.79 | 1.7 | 1.2 | 2.4 | 4.9 |
| CV% | 1.9 | 1.3 | 1.4 | 6.0 | 3.8 | 4.0 |
Recovery for Human PSG1 Quantikine ELISA Kit
The recovery of PSG-1 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 100 | 83-118 |
| EDTA Plasma (n=4) | 92 | 84-108 |
| Heparin Plasma (n=4) | 90 | 83-99 |
| Serum (n=4) | 91 | 81-104 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of PSG-1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human PSG1 Quantikine ELISA Kit
Human PSG-1 ELISA Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: PSG1
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional PSG1 Products
Product Documents for Human PSG1 Quantikine ELISA Kit
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human PSG1 Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Related Research Areas
Citations for Human PSG1 Quantikine ELISA Kit
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Protocols
View specific protocols for Human PSG1 Quantikine ELISA Kit (DPSG10):
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Human PSG1 Quantikine ELISA Kit
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Q: What is the normal appearance of Assay Diluent RD1-75?
A: Assay Diluent RD1-75 contains high concentrations of additives and typically has a very cloudy, brown appearance. It is recommended to mix this diluent well before and during use.