Human/Rat Neuroligin 2/NLGN2 Alexa Fluor™ Plus 680‑conjugated Antibody

R&D Systems | Catalog # AF5645AFP680

R&D Systems
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Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot

Label

Alexa Fluor Plus 680 (Excitation = 687 nm, Emission = 704 nm)

Antibody Source

Polyclonal Goat IgG
View all Neuroligin 2/NLGN2 Primary Antibodies »

Product Specifications

Specificity

Detects human and rat Neuroligin 2/NLGN2 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Neuroligin 2/NLGN2

Neuroligin 2 (NLGN2) is one of several type I transmembrane Neuroligins that are expressed on neuronal postsynaptic densities. Neuroligins play an important role in synaptic development and function (1). Mature human Neuroligin 2 is a 105 kDa protein that consists of a 663 amino acid (aa) extracellular domain (ECD) with a catalytically inactive cholinesterase-like domain, a 21 aa transmembrane segment, and a 137 aa cytoplasmic tail (2, 3). Within the ECD, human Neuroligin 2 shares 98% aa sequence identity with mouse and rat Neuroligin 2. Alternate splicing generates an isoform with a 17 aa insertion at splice site A within the ECD (2). This recombinant protein does not contain the splice site A insert (-SS A). Neuroligin 2 is expressed on neurons in the brain and also on pancreatic beta cells where it facilitates insulin secretion (2, 4, 5). The -SS A isoform is uniformly expressed among inhibitory and excitatory synapses, while the +SS A isoform is enriched at inhibitory GABAergic synapses (4, 6, 7). Neuroligin 2 clusters at postsynaptic densities in association with other postsynaptic proteins including S-SCAM, PSD-95, gephyrin, and Neuroligin 3 (4, 8 - 10). Synaptic maturation is promoted by the binding of Neuroligin 2 with presynaptic Neurexins, and these interactions are restricted to particular combinations of isoforms of the binding partners (11‑15). Neuroligin 2 interacts with the alpha and beta forms of Neurexin 1, 2, and 3 (14). Its -SS A and +SS A isoforms are bound equally well by Neurexin 1 beta isoforms (-SS4 or +SS4), although only the Neurexin 1 beta +SS4 isoform can induce development of Neuroligin 2‑dependent GABAergic contacts (7, 15).

References

  1. Sudhof, T.C. (2008) Nature 455:903.
  2. Ichtchenko, K. et al. (1996) J. Biol. Chem. 271:2676.
  3. Koehnke, J. et al. (2008) Proc. Natl. Acad. Sci. 105:1873.
  4. Varoqueaux, F. et al. (2004) Eur. J. Cell Biol. 83:449.
  5. Suckow, A.T. et al. (2008) Endocrinology 149:6006.
  6. Graf, E.R. et al. (2004) Cell 119:1013.
  7. Chih, B. et al. (2006) Neuron 51:171.
  8. Sumita, K. et al. (2007) J. Neurochem. 100:154.
  9. Irie, M. et al. (1997) Science 277:1511.
  10. Budreck, E.C. and P. Scheiffele (2008) Eur. J. Neurosci. 26:1738.
  11. Chih, B. et al. (2005) Science 307:1324.
  12. Chubykin, A.A. et al. (2007) Neuron 54:919.
  13. Hines, R.M., et al. (2008) J. Neurosci. 28:6055.
  14. Kang, Y. et al. (2008) J. Biol. Chem. 283:2323.
  15. Graf, E.R. et al. (2006) J. Neurosci. 26:4256.

Alternate Names

NLGN2

Entrez Gene IDs

57555 (Human); 216856 (Mouse); 117096 (Rat)

Gene Symbol

NLGN2

UniProt

EAW90197

Additional Neuroligin 2/NLGN2 Products

Product Documents

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

Related Research Areas

Neuronal Markers

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