Human/Rat Neuroligin 2/NLGN2 Antibody

Catalog # Availability Size / Price Qty
AF5645
AF5645-SP

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Detection of Human and Rat Neuroligin 2/NLGN2 by Western Blot.
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Human/Rat Neuroligin 2/NLGN2 Antibody Summary

Species Reactivity
Human, Rat
Specificity
Detects human and rat Neuroligin 2/NLGN2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human (rh) NGLN1, rhNGLN3, and rhNGLN4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Neuroligin 2/NLGN2
Gln15-Ser660
Accession # EAW90197
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1-3 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human and Rat Neuroligin 2/NLGN2 by Western Blot. View Larger

Detection of Human and Rat Neuroligin 2/NLGN2 by Western Blot. Western blot shows lysates of human cortex and rat cerebellum tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Rat Neuroligin 2/NLGN2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5645) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Neuroligin 2/NLGN2 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuroligin 2/NLGN2

Neuroligin 2 (NLGN2) is one of several type I transmembrane Neuroligins that are expressed on neuronal postsynaptic densities. Neuroligins play an important role in synaptic development and function (1). Mature human Neuroligin 2 is a 105 kDa protein that consists of a 663 amino acid (aa) extracellular domain (ECD) with a catalytically inactive cholinesterase-like domain, a 21 aa transmembrane segment, and a 137 aa cytoplasmic tail (2, 3). Within the ECD, human Neuroligin 2 shares 98% aa sequence identity with mouse and rat Neuroligin 2. Alternate splicing generates an isoform with a 17 aa insertion at splice site A within the ECD (2). This recombinant protein does not contain the splice site A insert (-SS A). Neuroligin 2 is expressed on neurons in the brain and also on pancreatic beta cells where it facilitates insulin secretion (2, 4, 5). The -SS A isoform is uniformly expressed among inhibitory and excitatory synapses, while the +SS A isoform is enriched at inhibitory GABAergic synapses (4, 6, 7). Neuroligin 2 clusters at postsynaptic densities in association with other postsynaptic proteins including S-SCAM, PSD-95, gephyrin, and Neuroligin 3 (4, 8 - 10). Synaptic maturation is promoted by the binding of Neuroligin 2 with presynaptic Neurexins, and these interactions are restricted to particular combinations of isoforms of the binding partners (11‑15). Neuroligin 2 interacts with the alpha and beta forms of Neurexin 1, 2, and 3 (14). Its -SS A and +SS A isoforms are bound equally well by Neurexin 1 beta isoforms (-SS4 or +SS4), although only the Neurexin 1 beta +SS4 isoform can induce development of Neuroligin 2‑dependent GABAergic contacts (7, 15).

References
  1. Sudhof, T.C. (2008) Nature 455:903.
  2. Ichtchenko, K. et al. (1996) J. Biol. Chem. 271:2676.
  3. Koehnke, J. et al. (2008) Proc. Natl. Acad. Sci. 105:1873.
  4. Varoqueaux, F. et al. (2004) Eur. J. Cell Biol. 83:449.
  5. Suckow, A.T. et al. (2008) Endocrinology 149:6006.
  6. Graf, E.R. et al. (2004) Cell 119:1013.
  7. Chih, B. et al. (2006) Neuron 51:171.
  8. Sumita, K. et al. (2007) J. Neurochem. 100:154.
  9. Irie, M. et al. (1997) Science 277:1511.
  10. Budreck, E.C. and P. Scheiffele (2008) Eur. J. Neurosci. 26:1738.
  11. Chih, B. et al. (2005) Science 307:1324.
  12. Chubykin, A.A. et al. (2007) Neuron 54:919.
  13. Hines, R.M., et al. (2008) J. Neurosci. 28:6055.
  14. Kang, Y. et al. (2008) J. Biol. Chem. 283:2323.
  15. Graf, E.R. et al. (2006) J. Neurosci. 26:4256.
Entrez Gene IDs
57555 (Human); 216856 (Mouse); 117096 (Rat)
Alternate Names
EC 3.1.1; EC 3.1.1.1; KIAA1366neuroligin-2; Neuroligin 2; NLGN2

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