Detection of Human and Rat Neuroligin 2/NLGN2 by Western Blot.
Western blot shows lysates of human cortex and rat cerebellum tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Rat Neuroligin 2/NLGN2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5645) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Neuroligin 2/NLGN2 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Neuroligin 2/NLGN2
Neuroligin 2 (NLGN2) is one of several type I transmembrane Neuroligins that are expressed on neuronal postsynaptic densities. Neuroligins play an important role in synaptic development and function (1). Mature human Neuroligin 2 is a 105 kDa protein that consists of a 663 amino acid (aa) extracellular domain (ECD) with a catalytically inactive cholinesterase-like domain, a 21 aa transmembrane segment, and a 137 aa cytoplasmic tail (2, 3). Within the ECD, human Neuroligin 2 shares 98% aa sequence identity with mouse and rat Neuroligin 2. Alternate splicing generates an isoform with a 17 aa insertion at splice site A within the ECD (2). This recombinant protein does not contain the splice site A insert (-SS A). Neuroligin 2 is expressed on neurons in the brain and also on pancreatic beta cells where it facilitates insulin secretion (2, 4, 5). The -SS A isoform is uniformly expressed among inhibitory and excitatory synapses, while the +SS A isoform is enriched at inhibitory GABAergic synapses (4, 6, 7). Neuroligin 2 clusters at postsynaptic densities in association with other postsynaptic proteins including S-SCAM, PSD-95, gephyrin, and Neuroligin 3 (4, 8 - 10). Synaptic maturation is promoted by the binding of Neuroligin 2 with presynaptic Neurexins, and these interactions are restricted to particular combinations of isoforms of the binding partners (11‑15). Neuroligin 2 interacts with the alpha and beta forms of Neurexin 1, 2, and 3 (14). Its -SS A and +SS A isoforms are bound equally well by Neurexin 1 beta isoforms (-SS4 or +SS4), although only the Neurexin 1 beta +SS4 isoform can induce development of Neuroligin 2‑dependent GABAergic contacts (7, 15).
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Varoqueaux, F. et al. (2004) Eur. J. Cell Biol. 83:449.
Suckow, A.T. et al. (2008) Endocrinology 149:6006.
Graf, E.R. et al. (2004) Cell 119:1013.
Chih, B. et al. (2006) Neuron 51:171.
Sumita, K. et al. (2007) J. Neurochem. 100:154.
Irie, M. et al. (1997) Science 277:1511.
Budreck, E.C. and P. Scheiffele (2008) Eur. J. Neurosci. 26:1738.
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