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Human/Rat PTK7/CCK4 APC-conjugated Antibody

Catalog #: FAB4499A Datasheet / COA / SDS

Discontinued Product

FAB4499A has been discontinued.
View all PTK7/CCK4 products.
Detection of PTK7/CCK4 in HepG2 Human Cell Line by Flow Cytometry.
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Human/Rat PTK7/CCK4 APC-conjugated Antibody Summary

Species Reactivity
Human, Rat
Specificity
Detects human and rat PTK7/CCK4 in Western blots.
Source
Monoclonal Mouse IgG1 Clone # 525222
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human PTK7/CCK4
Ala31-Ser199
Accession # Q13308
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of PTK7/CCK4 antibody in HepG2 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of PTK7/CCK4 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human/Rat PTK7/CCK4 APC-conjugated Monoclonal Antibody (Catalog # FAB4499A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: PTK7/CCK4

Protein tyrosine kinase 7 (PTK7), also known as colon carcinoma kinase 4 (CCK4), is a receptor tyrosine kinase (RTK) involved in colon carcinoma development and/or proliferation. Similar to the RTK HER3, PTK7 contains a catalytically inactive tyrosine kinase domain, suggesting a potential tumor-characteristic role as a signal amplifier or modulator for an as yet unidentified kinase-competent partner. PTK7 may also play a role during gastrulation in the rodent embryo.

Long Name
Protein Tyrosine Kinase 7
Entrez Gene IDs
5754 (Human); 71461 (Mouse); 301242 (Rat)
Alternate Names
CCK4; CCK-4; CCK4Colon carcinoma kinase 4; colon carcinoma kinase-4; Protein-tyrosine kinase 7; Pseudo tyrosine kinase receptor 7; PTK7 protein tyrosine kinase 7; PTK7; Tyrosine-protein kinase-like 7

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Product Datasheet
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SDS

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