Human ROBO4 PE-conjugated Antibody

(1 citations)   
  • Species Reactivity
  • Specificity
    Detects human ROBO4 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant rat ROBO1, recombinant human (rh) ROBO2, or rhROBO3 is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 265703
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human ROBO4
    Accession # Q8WZ75
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of ROBO4 in Human ROBO4 Transfectants by Flow Cytometry. Human ROBO4 transfectants were stained with Mouse Anti-Human ROBO4 PE‑conjugated Monoclonal Antibody (Catalog # FAB2454P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ROBO4
ROBO4, also known as Magic Roundabout, is a ~150 kDa glycoprotein belonging to the ROBO family (1). ROBOs are molecular guidance receptors that typically interact with Slit ligands to regulate axon guidance and neuronal migration (2). Unlike other family members, ROBO4 is mainly restricted to the vascular endothelium (1, 2). Expression in early hematopoietic progenitors is also reported (3). The human ROBO4 cDNA encodes 1012 amino acids (aa), including a 27 aa signal sequence, a 440 aa extracellular domain (ECD) containing two C2-type Ig domains and two fibronectin type III (FNIII) domains, a transmembrane domain and an intracellular domain. ROBO4 diverges from other ROBOs in the number of Ig, FNIII and cytoplasmic CC domains (1, 4). Within the ECD, human ROBO4 shares 80%, 80%, 87% and 88% aa identity with mouse, rat, bovine and canine ROBO4, respectively. Vascular endothelial ROBO4 is expressed at highest levels during development and vascular remodeling, including tumor angiogenesis (1, 2, 4-6). It is proposed to contribute to vascular stability. Consistent with this, endogenous ROBO4 is concentrated in the vascular stalk and sprouts rather than tip cells and appears to protect newly formed blood vessels against VEGF-induced vascular leak (6-9). ROBO4 binding of Slit proteins has been variably reported, and when detected may be mediated by ROBO4/ROBO1 heterodimers (2, 4-7, 9-13). ROBO4 is also variably reported to stimulate or inhibit cell migration or filopodia formation (2, 4-13). Effects on cell movement may be mediated through intracellular binding of WASP-, Ras/Rac/Rho-, Mena-, Src- or Paxillin-related proteins, all of which affect the cytoskeleton (5-7, 10-12). Recombinant soluble ROBO4 ECD can antagonize endothelial cell migration and in vivo angiogenesis (13). 
  • References:
    1. Huminiecki, L. et al. (2002) Genomics 79:547.
    2. Legg, J.A. et al. (2008) Angiogenesis 11:13.
    3. Shibata, F. et al. (2009) Stem Cells 27:183.
    4. Park, K.W. et al. (2003) Dev. Biol. 261:251.
    5. Seth, P. et al. (2005) Biochem. Biophys. Res. Commun. 332:533.
    6. Jones, C.A. et al. (2008) Nat. Med. 14:448.
    7. Jones, C.A. et al. (2009) Nat. Cell Biol. 11:1325.
    8. Chen, H. et al. (2010) Adv. Exp. Med. Biol. 664:457.
    9. London, N.R. et al. (2009) J. Thromb. Haemost. 7:57.
    10. Verissimo, A.R. et al. (2009) Biochem. Soc. Trans. 37:1214.
    11. Sheldon, H. et al. (2009) FASEB J. 23:513.
    12. Kaur, S. et al. (2008) BMC Cell Biol. 9:61.
    13. Suchting, S. et al. (2005) FASEB J. 19:121.
  • Long Name:
    Roundabout Homolog 4
  • Entrez Gene IDs:
    54538 (Human); 74144 (Mouse)
  • Alternate Names:
    ECSM4; FLJ20798; Magic roundabout; MGC133352; MGC133353; MRB; roundabout homolog 4; roundabout homolog 4, magic roundabout (Drosophila)
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 1 of 1

  1. The impact of proliferative potential of umbilical cord-derived endothelial progenitor cells and hypoxia on vascular tubule formation in vitro.
    Authors: Zhang Y, Fisher N, Newey SE
    Stem Cells Dev., 2009;18(2):359-75.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
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Mouse IgG2A PE-conjugated Antibody

Ctrl IC003P 10  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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