Human S1P2/EDG-5 Antibody

R&D Systems | Catalog # MAB3649

R&D Systems
Discontinued Product
MAB3649 has been discontinued. View all S1P2/EDG-5 products.

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 368510
View all S1P2/EDG-5 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human S1P2/EDG-5
Met1-Val353
Accession # AAC98919

Specificity

Detects human S1P

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human S1P2/EDG-5 Antibody

Detection of S1P2/EDG-5 antibody in HEK293 Human Cell Line Transfected with Human S1P2/EDG-5 and eGFP antibody by Flow Cytometry.

Detection of S1P2/EDG-5 in HEK293 Human Cell Line Transfected with Human S1P2/EDG-5 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either (A) human S1P2/EDG-5 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human S1P2/EDG-5 Monoclonal Antibody (Catalog # MAB3649) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

Applications for Human S1P2/EDG-5 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with human S1P2/EDG-5 and eGFP

Reviewed Applications

Read 1 review rated 3 using MAB3649 in the following applications:

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: S1P2/EDG-5

S1P2 (sphingosine 1-phosphate receptor-2), also known as EDG-5 (endothelial differentiation, G-protein coupled receptor-5), is a 353 amino acid (aa) seven-transmembrane receptor putative glycoprotein that binds the lysolipid phosphoric acid mediator, sphingosine 1-phosphate. Extracellular portions of human S1P2 show 82.5% aa identity with mouse S1P2 and 92% aa identity with human EDG-3, the most closely related family member. Both are expressed predominantly in the lung, heart, kidney, liver, spleen, thymus, testis and brain, mediating both mitogenic and anti-apoptotic effects.

Long Name

Endothelial Differentiation Gene 5

Alternate Names

AGR16, EDG5, GPCR13, GPCR18, H218, LPB2, S1PR2

Entrez Gene IDs

9294 (Human); 14739 (Mouse); 29415 (Rat)

Gene Symbol

S1PR2

UniProt

AAC98919

Additional S1P2/EDG-5 Products

Product Documents for Human S1P2/EDG-5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human S1P2/EDG-5 Antibody

For research use only

Citations for Human S1P2/EDG-5 Antibody

Customer Reviews for Human S1P2/EDG-5 Antibody (1)

3 out of 5
1 Customer Rating
5 Stars
0%
4 Stars
0%
3 Stars
100%
2 Stars
0%
1 Stars
0%

Have you used Human S1P2/EDG-5 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Human S1P2/EDG-5 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Whole blood cells
    Species: Canine
    Verified Customer | Posted 10/12/2018
    Canine IP
    Human S1P2/EDG-5 Antibody MAB3649

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies