Detection of S1P2/EDG-5 in HEK293 Human Cell Line Transfected with Human S1P2/EDG-5 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human S1P2/EDG-5 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human S1P2/EDG-5 Monoclonal Antibody (Catalog # MAB3649) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
S1P2 (sphingosine 1-phosphate receptor-2), also known as EDG-5 (endothelial differentiation, G-protein coupled receptor-5), is a
353 amino acid (aa) seven-transmembrane receptor putative glycoprotein
that binds the lysolipid phosphoric acid mediator, sphingosine
1-phosphate. Extracellular portions of human S1P2 show 82.5% aa
identity with mouse S1P2 and 92% aa identity with human EDG-3, the
most closely related family member. Both are expressed predominantly in
the lung, heart, kidney, liver, spleen, thymus, testis and brain,
mediating both mitogenic and anti-apoptotic effects.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.