Detection of S1P2/EDG-5 in HEK293 Human Cell Line Transfected with Human S1P2/EDG-5 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with either (A) human S1P2/EDG-5 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human S1P2/EDG-5 Monoclonal Antibody (Catalog # MAB3649) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
S1P2 (sphingosine 1-phosphate receptor-2), also known as EDG-5 (endothelial differentiation, G-protein coupled receptor-5), is a
353 amino acid (aa) seven-transmembrane receptor putative glycoprotein
that binds the lysolipid phosphoric acid mediator, sphingosine
1-phosphate. Extracellular portions of human S1P2 show 82.5% aa
identity with mouse S1P2 and 92% aa identity with human EDG-3, the
most closely related family member. Both are expressed predominantly in
the lung, heart, kidney, liver, spleen, thymus, testis and brain,
mediating both mitogenic and anti-apoptotic effects.
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