Key Product Details

Species Reactivity

Mouse

Applications

Control

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 11711
View all IgG1 Isotype Controls »

Product Specifications

Immunogen

KLH

Specificity

Selected for use as mouse IgG1 isotype control. In direct ELISAs, does not cross-react with recombinant human (rh) 6Ckine, recombinant mouse (rm) 6Ckine, rhACT-II, rhBLC/BCA-1, rmBLC, rmC10, recombinant rat (rr) CINC-1, rrCINC-2 alpha, rrCINC-2 beta, rrCINC-3, rhCK beta 8-1, recombinant viral (rv) CMV UL146, rmCRG-2, rhENA-78, rhEotaxin, rmEotaxin, rhEotaxin-2, rmEotaxin-2, rhEotaxin-3, rhFractalkine, rmFractalkine, rrFractalkine, rhGCP-2, rmGCP-2, rhGRO alpha, rhGRO beta, rhGRO gamma, rhHCC-1, rhI-309, rhI-Linck, rhIL-8, recombinant porcine IL-8, rhIP-10, rhI-TAC, rmJE, rmKC, rhLeukotactin-1, rhLymphotactin, rmLymphotactin, rmMARC, rhMCP-1, rhMCP-2, rhMCP-3, rhMCP-4,rmMCP-5, rvMCV type 2, rhMDC, rmMDC, rhMIG, rmMIG, rhMIP-1 alpha, rmMIP-1 alpha, rmMIP-1 beta, rhMIP-1 delta, rmMIP-1 gamma, rmMIP-2, rhMIP-3 alpha,rmMIP-3 alpha, rrMIP-3 alpha, rhMIP-3 beta, rmMIP-3 beta, rvMIP-I, rvMIP-II, rvMIP-III, rhMPIF-1, rhNAP-2, rhPARC, rhRANTES, rmRANTES, rhSDF-1 alpha, rmSDF-1 alpha, rhSDF-1 beta, rhTARC, rmTARC, rmTCA-3, rhTeck, or rmTeck.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse IgG1 Isotype Control

Detection of Mouse IgG1Isotype Isotype Control by Flow Cytometry

Detection of Mouse IgG1Isotype Control by Flow Cytometry

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human LILRA6/CD85b Monoclonal Antibody (Catalog # MAB8656, filled histogram) or Mouse IgG1Isotype Control Antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Detection of Human IgG1 by Flow Cytometry

Detection of Human IgG1 by Flow Cytometry

EC and PC ICAM-1 expression and effects of CD18 blocking on neutrophil adhesion.ICAM-1 expression on (A) EC or (D) PC monolayers before and after 4 hr IL-1 beta activation was assessed by flow cytometry (IgG labeled control white). ICAM-1 expression on (B,C) EC and (E,F) PC following 4 hr IL-1 beta activation was imaged using confocal microscopy. Scale bars are 20 µm. (G) Neutrophil adhesion inhibition to IL-1 beta activated EC or PC monolayers. Freshly isolated neutrophils were pre-incubated with anti-Mac-1 or anti-LFA-1 antibodies and seeded onto EC or PC monolayers (activated for 4 with IL-1 beta ) in Sykes-Moore chambers and allowed to adhere prior to counting. Bars represent average neutrophil adhesion ± SEM. *P<0.05, when compared to the no block control. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0060025), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human IgG1 by Flow Cytometry

Detection of Human IgG1 by Flow Cytometry

EC and PC ICAM-1 expression and effects of CD18 blocking on neutrophil adhesion.ICAM-1 expression on (A) EC or (D) PC monolayers before and after 4 hr IL-1 beta activation was assessed by flow cytometry (IgG labeled control white). ICAM-1 expression on (B,C) EC and (E,F) PC following 4 hr IL-1 beta activation was imaged using confocal microscopy. Scale bars are 20 µm. (G) Neutrophil adhesion inhibition to IL-1 beta activated EC or PC monolayers. Freshly isolated neutrophils were pre-incubated with anti-Mac-1 or anti-LFA-1 antibodies and seeded onto EC or PC monolayers (activated for 4 with IL-1 beta ) in Sykes-Moore chambers and allowed to adhere prior to counting. Bars represent average neutrophil adhesion ± SEM. *P<0.05, when compared to the no block control. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0060025), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human IgG1 by Control

Detection of Human IgG1 by Control

miR-720 maintains the migratory and invasive properties of MDA-MB-231 cells. MDA-MB-231 cells were transfected with 50 nM anti-miR-720 or anti-miR-negative control (Ctrl) for 48 h. Either RNA was isolated (a) or transfected cells subjected to functional assays (b–e). a miR-720 levels. RNA was subjected to RT-qPCR for miR-720. Control condition (Ctrl) was set to 1.0 (mean ± SD from three independent experiments). b Migration assay. Transfected cells were subjected to a migration assay in a Boyden chamber over a 24-h period. The cells that migrated through the chamber were quantified using crystal violet staining. Ctrl was set to 100 % (mean ± SD from three independent experiments). c Matrigel assay. Transfected cells were grown in Matrigel for 11–15 days and then photographed using a Nikon eclipse TS100 microscope at 10× magnification. Scale bar = 100 μm. Representative images of two independent experiments with similar results are shown (left panels). The number of branched colonies were counted. The count in the control sample was set to 1.0 (right panel). d Transendothelial migration assay. Transfected MDA-MB-231 cells were plated in a Boyden chamber that had been coated with a confluent monolayer of HUVEC cells, and the cancer cells that invaded through the HUVEC layer over a 24-h period were quantified using crystal violet staining. Ctrl value for transendothelial (transendo.) migration was set to 100 % (mean ± SD from three independent experiments) and relative (Rel.) migration values given. e ATP assay. Transfected cells were grown for 24 h and ATP levels were measured using an ATPlite 1Step assay. Ctrl was set to 100 % (mean ± SD from three independent experiments). *P < 0.05, ***P < 0.001, Student’s t test. Not sig. not significant Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27039296), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Mouse IgG1 Isotype Control by Flow Cytometry

Detection of Mouse Mouse IgG1 Isotype Control by Flow Cytometry

Monoclonal ASM antibody (23A12C3) inhibits pathogenic Th17 cell differentiation and protects neuropathological features in APP/PS1 mice. e Representative flow cytometry plot and graph displaying the calculated percentage and mean fluorescent intensity of Th17 cells (CD4+ IL17A+) differentiated from CD4+ T cell in each group (Control, n = 5; Smpd1ox/ox serum/con IgG ab, n = 10; Smpd1ox/ox serum/23A12C3, n = 8). f Western blotting for p-Stat3, p-JNK, p-AKT, p-mTOR in Th17 cells differentiated from CD4+ T cell in each group (n = 4/group). g ASM activity in plasma (n = 5/group). h Percentage of Th17 cells in the spleen, blood, and brain by flow cytometry (n = 5/group). i Quantification of microglia (Iba-1) and astrocyte (GFAP) (n = 5/group). j mRNA levels of inflammatory markers in the cortex and hippocampus (n = 4/group). k Representative immunofluorescence images and quantification of ThioS (A beta plaques) in cortex and hippocampus. Scale bars, 50 μm. (n = 5/group). l Quantification of 6E10 (n = 5/group). m The results of Morris water maze test (n = 10/group). n, o At probe trial day 11, time spent in target platform (n) and the number of times each animal entered the small target zone (o) were analyzed (n = 10/group). p The results of fear-conditioning test (n = 10/group). d–j, m–p One-way analysis of variance, Tukey’s post hoc test. k, l, n Two-tailed student’s t test. All error bars indicate s.e.m. All in vivo data analysis was done at 9-mo-old mice. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/36959217), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse IgG1 Isotype Control

Application
Recommended Usage

Control

Negative control for use in conjunction with R&D Systems antibodies. Use at the same concentration as the detection antibody.

Reviewed Applications

Read 24 reviews rated 4.7 using MAB002 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IgG1

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G1

Alternate Names

IgG1, Igh-4, RGD1359539, VH7183

Additional IgG1 Products

Product Documents for Mouse IgG1 Isotype Control

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse IgG1 Isotype Control

For research use only

Citations for Mouse IgG1 Isotype Control

Customer Reviews for Mouse IgG1 Isotype Control (24)

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24 Customer Ratings
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Customer Images

Showing  1 - 5 of 24 reviews Showing All
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  • Mouse IgG1 Isotype Control
    Name: Anonymous
    Application: Cell culture treatment
    Sample Tested: MCF-7 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 05/30/2025
    MCF7 were seeded in 96well plates at 5000 cels/well with 10 ug/mL of antibody.
    Mouse IgG1 Isotype Control MAB002
  • Mouse IgG1 Isotype Control
    Name: Anonymous
    Application: Cell culture treatment - 5000 cells were treated with 10 ug/mL of antibody
    Sample Tested: HCT-116 human colorectal carcinoma cell line
    Species: Human
    Verified Customer | Posted 05/26/2025
    Mouse IgG1 Isotype Control MAB002
  • Mouse IgG1 Isotype Control
    Name: Lu Bai
    Application: Flow Cytometry
    Sample Tested: Dendritic cells
    Species: Human
    Verified Customer | Posted 10/11/2022
  • Mouse IgG1 Isotype Control
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum
    Species: Human
    Verified Customer | Posted 08/18/2021
    Mouse IgG1 Isotype Control MAB002
  • Mouse IgG1 Isotype Control
    Name: Anonymous
    Application: Block/Neutralize
    Sample Tested: A549 human lung carcinoma cell line
    Species: Human
    Verified Customer | Posted 05/05/2021
    Mouse IgG1 Isotype Control MAB002
  • Mouse IgG1 Isotype Control
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Peripheral blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 01/24/2021
    Mouse IgG1 Isotype Control MAB002
  • Mouse IgG1 Isotype Control
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: K562 human chronic myelogenous leukemia cell line
    Species: Human
    Verified Customer | Posted 10/06/2020
    Mouse IgG1 Isotype Control MAB002
  • Mouse IgG1 Isotype Control
    Name: Anonymous
    Application: ELISA
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 07/08/2020
    used as a negative control
    Mouse IgG1 Isotype Control MAB002
  • Mouse IgG1 Isotype Control
    Name: Abby Sukarto
    Application: Flow Cytometry
    Sample Tested: Bone Extracts
    Species: Human
    Verified Customer | Posted 02/13/2020
    Mouse IgG1 Isotype Control MAB002
  • Mouse IgG1 Isotype Control
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Mouse Breast Cancer Cells
    Species: Mouse
    Verified Customer | Posted 01/15/2020
  • Mouse IgG1 Isotype Control
    Name: Anonymous
    Application: Block/Neutralize
    Sample Tested: SVEC4-10 mouse vascular endothelial cell line
    Species: Mouse
    Verified Customer | Posted 01/06/2020
  • Mouse IgG1 Isotype Control
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Mouse breast cancer cell line
    Species: Mouse
    Verified Customer | Posted 12/08/2019
  • Mouse IgG1 Isotype Control
    Name: Abby Sukarto
    Application: Flow Cytometry
    Sample Tested: Bone Extracts
    Species: Mouse and Human
    Verified Customer | Posted 09/10/2019
    Need optimization of your specific cell types and use the same amount as the primary antibody.
  • Mouse IgG1 Isotype Control
    Name: Anonymous
    Application: Affinity Purification
    Sample Tested: Purified protein
    Species: Human
    Verified Customer | Posted 08/09/2019
    Mouse IgG1 Isotype Control MAB002
  • Mouse IgG1 Isotype Control
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: SK-BR-3 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 10/23/2018
    Mouse IgG1 Isotype Control MAB002
  • Mouse IgG1 Isotype Control
    Name: Anonymous
    Application: Block/Neutralize
    Sample Tested: Breast cancer cells
    Species: Human
    Verified Customer | Posted 05/20/2018
    We used this as an Isotype Control for our Endothelial Chemotaxis Assay experiments. Conditioned Medium from Breast cancer cells mixed with IgG1 Isotype Control was used to compare Conditioned Medium containing cytokines (IL6, IL8, IL1B) neutralization antibody cocktail. Cancer Res; 78(5) March 1, 2018 (Fig 6F)
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 03/23/2018
  • Mouse IgG1 Isotype Control
    Name: Tao Huang
    Application: Block/Neutralize
    Sample Tested: IgG1
    Species: Mouse
    Verified Customer | Posted 10/20/2017
  • Mouse IgG1 Isotype Control
    Name: Anonymous
    Application: Block/Neutralize
    Sample Tested: Blood-derived monocytes
    Species: Human
    Verified Customer | Posted 08/01/2017
  • Mouse IgG1 Isotype Control
    Name: A G
    Verified Customer | Posted 05/26/2017
    Mouse IgG1 Isotope control for ICC
    Used in ICC with dog blood cell on a slide.
    Mouse IgG1 Isotype Control MAB002
  • Mouse IgG1 Isotype Control
    Name: Qing Zhang
    Application: Immunoprecipitation
    Sample Tested: Cancer cell lysates
    Species: Human
    Verified Customer | Posted 05/16/2017
  • Mouse IgG1 Isotype Control
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Beta TC-6 mouse beta cell insulinoma cell line
    Species: Human and Mouse
    Verified Customer | Posted 08/17/2016
    PFA 4% fixation. Used at 94ug/mL. No signal until 120-250msec of acquisition time.
  • Mouse IgG1 Isotype Control
    Name: Anonymous
    Application: Functional Assay
    Sample Tested: uman primary monocytes
    Species: Human
    Verified Customer | Posted 04/21/2016
  • Mouse IgG1 Isotype Control
    Name: SOjan Abraham
    Application: Flow Cytometry
    Sample Tested: Peripheral blood lymphocytes (PBL)
    Species: Human
    Verified Customer | Posted 01/21/2016

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