Human sFRP‑5 Alexa Fluor™ Plus 647‑conjugated Antibody

R&D Systems | Catalog # FAB6266AFP647

R&D Systems
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Key Product Details

Species Reactivity

Human

Applications

Neutralization

Label

Alexa Fluor Plus 647 (Excitation = 658 nm, Emission = 675 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 707128
View all sFRP-5 Primary Antibodies »

Product Specifications

Specificity

Detects human sFRP-5 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human sFRP‑5 Alexa Fluor™ Plus 647‑conjugated Antibody

Application
Recommended Usage

Neutralization

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: sFRP-5

Secreted Frizzled Related Protein-5 (sFRP-5), also known as SARP-3, belongs to a family of Wnt‑binding proteins with homology to the ligand‑binding domain of the Frizzled receptors. sFRPs are approximately 30-35 kDa in size and contain an N‑terminal Frizzled-like domain with 10 conserved cysteines and a Netrin-like C-terminal domain (1-3). Mature human sFRP-5 shares 96% aa sequence identity with mouse and rat sFRP-2 (4). During embryonic development, sFRP-5 is expressed in the anterior visceral endoderm, neural tube, foregut epithelium, and proliferating and prehypertrophic chondrocytes (5-8). sFRP-5 activity is required for the development of foregut, liver, ventral pancreas, and somites (6, 7). In the adult, sFRP-5 is expressed in the retinal pigment epithelium and pancreas (4, 9). sFRP-5 binds and antagonizes the function of mammalian Wnt-5a and Wnt-11 as well as Xenopus Xwnt-8, resulting in an inhibition of both canonical and non-canonical Wnt signaling (7, 9, 10). sFRP-5 down-regulation is common in breast and gastric cancer cells and is correlated with poor prognosis (11-13). It functions as a tumor suppressor by inhibiting epithelial-mesenchymal transition, invasiveness, and tumorigenicity of ovarian cancer cells (14). sFRP-5 plays an important role in maintaining glucose handling and insulin sensitivity (10). It is secreted by adipocytes and is down-regulated in mouse models of obesity and type 2 diabetes (10).

References

  1. Bovolenta, P. et al. (2008) J. Cell Sci. 121:737.
  2. van Amerongen, R. and R. Nusse (2009) Development 136:3205.
  3. Rattner, A. et al. (1997) Proc. Natl. Acad. Sci. 94:2859.
  4. Melkonyan, H.S. et al. (1997) Proc. Natl. Acad. Sci. 94:13636.
  5. Leaf, I. et al. (2006) Genesis 44:573.
  6. Satoh, W. et al. (2008) Genesis 46:92.
  7. Li, Y. et al. (2008) Genes Dev. 22:3050.
  8. Witte, F. et al. (2009) Gene Expr. Patterns 9:215.
  9. Chang, J. et al. (1999) Hum. Mol. Genet. 8:575.
  10. Ouchi, N. et al. (2010) Science 329:454.
  11. Veeck, J. et al. (2008) Carcinogenesis 29:991.
  12. Zhao, C. et al. (2009) BMC Cancer 9:224.
  13. Ho, C.M. et al. (2010) Eur. J. Clin. Invest. 40:310.
  14. Su, H.Y. et al. (2010) Int. J. Cancer 127:555.

Long Name

Secreted Frizzled Related Protein 5

Alternate Names

FRP1B, SARP3, sFRP5

Entrez Gene IDs

6425 (Human); 54612 (Mouse)

Gene Symbol

SFRP5

UniProt

Q5T4F7

Additional sFRP-5 Products

Product Documents for Human sFRP‑5 Alexa Fluor™ Plus 647‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human sFRP‑5 Alexa Fluor™ Plus 647‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

Related Research Areas

Wnt Family
Wnt Inhibitors

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