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Human SOX3 Antibody

Catalog #: MAB2569 1 Review Datasheet / COA / SDS

Discontinued Product

MAB2569 has been discontinued.
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SOX3 in NTera‑2 Human Cell Line.
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SOX3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human SOX3 in direct ELISAs. In direct ELISAs, no cross‑reactivity with recombinant human SOX2, 7, 10, or 17 is observed.
Source
Monoclonal Mouse IgG2A Clone # 287403
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human SOX3
Met1-Leu230
Accession # P41225
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry SOX3 antibody in NTera-2 Human Cell Line by Immunocytochemistry (ICC). View Larger

SOX3 in NTera‑2 Human Cell Line. SOX3 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line differentiation with retinoic acid using Mouse Anti-Human SOX3 Monoclonal Antibody (Catalog # MAB2569) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green, upper panel; Catalog # NL009) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOX3

SOX3 belongs to the Sox (SRY-like HMG-box gene) family of transcription factors with diverse roles in development. SOX3 is expressed in the central nervous system from the earliest stages of development. It is one of the earliest neural markers in vertebrates and plays a role in specifying neuronal fate.

Long Name
Transcription Factor SOX3
Entrez Gene IDs
6658 (Human)
Alternate Names
GHDX; MRGH; panhypopituitarism; PHP; PHPX; SOX3; SOXB; SRY (sex determining region Y)-box 3; transcription factor SOX-3

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Human SOX3 Antibody
By Anonymous on 01/04/2022
Application: Immunocytochemistry/Immunofluorescence Sample Tested: U87 glioma cells Species: Human
Immunocytochemistry/Immunofluorescence Human SOX3 Antibody MAB2569