Human SOX3 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human SOX3 in direct ELISAs. In direct ELISAs, no cross‑reactivity with recombinant human SOX2, 7, 10, or 17 is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 287403
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human SOX3
    Met1-Leu230
    Accession # P41225
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
SOX3 in NTera‑2 Human Cell Line. SOX3 was detected in immersion fixed NTera‑2 human testicular embryonic carcinoma cell line differentiation with retinoic acid using Mouse Anti-Human SOX3 Monoclonal Antibody (Catalog # MAB2569) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green, upper panel; Catalog # NL009) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SOX3
SOX3 belongs to the Sox (SRY-like HMG-box gene) family of transcription factors with diverse roles in development. SOX3 is expressed in the central nervous system from the earliest stages of development. It is one of the earliest neural markers in vertebrates and plays a role in specifying neuronal fate.
  • Long Name:
    Transcription Factor SOX3
  • Entrez Gene IDs:
    6658 (Human)
  • Alternate Names:
    GHDX; MRGH; panhypopituitarism; PHP; PHPX; SOX3; SOXB; SRY (sex determining region Y)-box 3; transcription factor SOX-3
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