Human STRO-1 Alexa Fluor® 647-conjugated Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of STRO-1 in MG-63 Cell Line by Flow Cytometry. Human MG-63 osteosarcoma cell line was stained with Mouse Anti-Human STRO-1 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB1038R, filled histogram) or Mouse IgM isotype control antibody (open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of STRO-1 in Human Mesenchymal Stem Cells by Flow Cytometry. Human mesenchymal stem cells were stained with (A) Mouse Anti-Human STRO-1 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB1038R, filled histogram) or (B) Mouse IgM isotype control antibody and Mouse anti-Human CD90 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB2067G). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
STRO-1 is a cell surface protein expressed by bone marrow stromal cells and erythroid precursors. The frequency of colony forming units-fibroblasts (CFU-F) was enriched 100-fold in the STRO-1+/Glycophorin A- population from bone marrow cells (1). The subset of marrow cells that expresses the STRO-1 antigen is capable of differentiating into multiple mesenchymal lineages including hematopoiesis-supportive stromal cells with a vascular smooth muscle-like phenotype, adipocytes, osteoblasts, and chondrocytes (2).
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
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