Human Syntaxin 16 Alexa Fluor® 405-conjugated Antibody

Catalog #: AF5648V Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5648V-100UG

Save 15% on Select RUO Reagents. See Details.

All Syntaxin 16 Antibodies

Product Details

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Syntaxin 16 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Syntaxin 16 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human (rh) Syntaxin 1A, rhSyntaxin 6, and rhSyntaxin BP1 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Syntaxin 16 isoform B
Leu165-Lys301
Accession # O14662

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Syntaxin 16

Syntaxin 16 (STX16; also SNY16) is a 39 kDa member of the syntaxin family of proteins. It is ubiquitously expressed, embedded in the Golgi membrane, and participates in the fusion of early endosomes with the Golgi stacks. Syntaxin 16 contributes one of four coiled-coil domains necessary for retrograde transport. Human Syntaxin 16 is a type IV single-pass transmembrane protein (very short lumenal C-terminus) that is 325 amino acids (aa) in length. It contains a cytoplasmic syntaxin region (aa 74‑180), a coiled-coil region (aa 230‑292), and a short, three amino acid C‑terminal lumenal sequence. There are multiple potential isoforms. Two show alternate start sites at Met187 and Met54, while three others show deletions of aa 45‑48, 28‑48 and 28‑44, respectively. A cytoplasmic form shows an Ala substitution for aa 132‑325. Over aa 165‑301, human Syntaxin 16 shares 95% aa identity with mouse Syntaxin 16.

Entrez Gene IDs

8675 (Human); 228960 (Mouse); 362283 (Rat)

Alternate Names

MGC90328; STX16; Syn16; SYN16hsyn16; Syntaxin 16; syntaxin-16