Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Syntaxin 16 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Syntaxin 16 isoform B
Leu165-Lys301
Accession # O14662

Specificity

Detects human Syntaxin 16 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human (rh) Syntaxin 1A, rhSyntaxin 6, and rhSyntaxin BP1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Syntaxin 16 Antibody

Detection of Human Syntaxin 16 antibody by Western Blot.

Detection of Human Syntaxin 16 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Syntaxin 16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5648) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Syntaxin 16 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human Syntaxin 16 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Syntaxin 16

Syntaxin 16 (STX16; also SNY16) is a 39 kDa member of the syntaxin family of proteins. It is ubiquitously expressed, embedded in the Golgi membrane, and participates in the fusion of early endosomes with the Golgi stacks. Syntaxin 16 contributes one of four coiled-coil domains necessary for retrograde transport. Human Syntaxin 16 is a type IV single-pass transmembrane protein (very short lumenal C-terminus) that is 325 amino acids (aa) in length. It contains a cytoplasmic syntaxin region (aa 74‑180), a coiled-coil region (aa 230‑292), and a short, three amino acid C‑terminal lumenal sequence. There are multiple potential isoforms. Two show alternate start sites at Met187 and Met54, while three others show deletions of aa 45‑48, 28‑48 and 28‑44, respectively. A cytoplasmic form shows an Ala substitution for aa 132‑325. Over aa 165‑301, human Syntaxin 16 shares 95% aa identity with mouse Syntaxin 16.

Alternate Names

STX16, Syn16

Entrez Gene IDs

8675 (Human); 228960 (Mouse); 362283 (Rat)

Gene Symbol

STX16

UniProt

O14662

Additional Syntaxin 16 Products

Product Documents for Human Syntaxin 16 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Syntaxin 16 Antibody

For research use only

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