Human TIMP Luminex® Performance Assay 3-plex Fixed Panel

For the measurement of TIMP-1, TIMP-2, and TIMP-4. Earn double rewards for reviews
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LKTM003

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Human TIMP Luminex Panel Standard Curves
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Product Details
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Human TIMP Luminex® Performance Assay 3-plex Fixed Panel Summary

Panel Luminex Performance Human Fixed TIMP Panel
Assay Type Magnetic bead-based multiplex assay for the Luminex® platform
Format 1 x 96-well microplate and Magnetic antibody-coated beads
Species Human
Analytes Detected Please see analyte list in assay customization tool below.
Performance Validation Our Luminex High Performance Assays undergo our most extensive validation testing. All Luminex High Performance Assay Panels are validated for use with serum and plasma samples. Some High Performance Assays are additionally validated for cell culture supernatants, milk, saliva, or urine, as indicated on the individual product data sheets. Luminex High Performance Assays are tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types. Recovery values for individual samples in validated sample types are also tested. Validation data for each analyte can be found in the product datasheet.

Analytes Included in the Assay
TIMP-1
TIMP-2
TIMP-4
Information for Compatible Analytes

For performance data for the different analyte-specific beads, please see product datasheets for the compatible individual Bead Sets.

Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Customization Tool. We also offer Custom Luminex Services.

Product Features

Magnetic Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples.

  • Simultaneously profile analytes in one sample
  • Magnetic format allows for easier wash steps
  • Undergoes similar validation testing as Quantikine® ELISA Kits
  • Precise and Specific
  • Requires a small sample volume (<50 µL)
  • Run your assay in just 3-5 hours
  • Mass-calibrated standards for consistent results with every new lot of material
  • Enough reagents for one 96-well plate
Kit Components
  • Premixed cocktail of antibody-coated Magnetic beads
  • Premixed cocktail of biotinylated detection antibody
  • Standard Cocktail(s)
  • Bead Diluent
  • Biotin Antibody Diluent
  • Calibrator Diluent
  • Wash Buffer
  • Streptavidin-PE
  • One flat-bottom 96-well Microplate
  • Foil Plate Sealers (4)
  • Mixing Bottle
  • Standard Value Card**

**A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Kit.

Precision

Intra-Assay Precision - is generated from the mean of the %CV's from 40 reportable results across two different concentrations of analytes in a single serum assay

Inter-Assay Precision - is generated from the mean of the %CV's across two different concentrations of analytes across 30 different serum assays.

  n=40 (20x2 levels) n=30
Analyte Intra-Assay (%CV) Inter-Assay (%CV)
TIMP-1 7.0 11.2
TIMP-2 4.3 9.9
TIMP-4 5.8 10.8

Accuracy

Linearity - The data represents mean spiked or natural linearity in serum matrix samples. Samples with natural linearity are marked with an asterisk.

Spike Recovery - The data represents mean percent recovery of spiked standards ranging from low, medium, and high concentration in serum matrix samples.

  Linearity Spiked
Recovery
Analyte Average % Average %
TIMP-1 111 NA
TIMP-2 100 84
TIMP-4 88 94
NA = Not applicable
Which Luminex Assay is right for me?

Assays for the Luminex platform are offered as High Performance Assays or Assays. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays.

More Information About Magnetic Bead-based Multiplex Kits For The Luminex® Platform

Magnetic Luminex High Performance Assays are designed for use with the Luminex MAGPIX CCD Imager. Alternatively, kits can be used with the Luminex 100/200™ or FLEXMAP 3D, dual laser, flow-based sorting and detection platforms.

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. One laser is bead-specific and determines which analyte is being detected. A magnet in the analyzer captures and holds the superparaMagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a Bio-Rad Bio-Plex dual laser, flow-based systems.

Scientific Data

Human TIMP Luminex Panel Standard Curves

Human TIMP Performance Luminex Panel Serum Sample Values Serum samples from 24 apparently healthy individuals were run on the Human TIMP Luminex High Performance Panel and sample values plotted. Minimum and maximum values shown by exterior lines, the box represents the 1st and 3rd quartiles with the center line representing the median.

Background: TIMP Assay Kits

TIMPs-1 through -4 regulate the activity of zinc metalloproteases known as MMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin- like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation of MMP-2 by MMP-14.

Long Name
Tissue Inhibitors of Metalloproteinases 1
Alternate Names
TIMP Assay Kits

Product Datasheets

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Product Specific Notices
Luminex® is a registered trademark of Luminex Corporation.

Citations for Human TIMP Luminex® Performance Assay 3-plex Fixed Panel

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Anethole induces anti-oral cancer activity by triggering apoptosis, autophagy and oxidative stress and by modulation of multiple signaling pathways
    Authors: C Contant, M Rouabhia, L Loubaki, F Chandad, A Semlali
    Scientific Reports, 2021-06-22;11(1):13087.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Markers of tissue remodeling and inflammation in the white and brown adipose tissues of a model hibernator
    Authors: SM Logan, KB Storey
    Cellular Signalling, 2021-03-09;82(0):109975.
    Species: Squirrel
    Sample Types: Tissue Homogenates
  3. Plasma levels of C-reactive protein, matrix metalloproteinase-7 and lipopolysaccharide-binding protein distinguish active pulmonary or extrapulmonary tuberculosis from uninfected controls in children
    Authors: VVS Albuquerqu, NP Kumar, KF Fukutani, B Vasconcelo, MB Arriaga, PS Silveira-M, S Babu, BB Andrade
    Cytokine, 2019-07-09;123(0):154773.
    Species: Human
    Sample Types: Plasma
  4. Adipose-derived stromal cell secretome reduces TNF?-induced hypertrophy and catabolic markers in primary human articular chondrocytes
    Authors: S Niada, C Giannasi, M Gomarasca, D Stanco, S Casati, AT Brini
    Stem Cell Res, 2019-05-15;38(0):101463.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Effects of aqueous suppressants and prostaglandin analogues on early wound healing after glaucoma implant surgery
    Authors: KI Jung, JE Woo, CK Park
    Sci Rep, 2019-03-27;9(1):5251.
    Species: Rabbit
    Sample Types: Aqueous Humor
  6. Heme Oxygenase-1 Regulation of Matrix Metalloproteinase-1 Expression Underlies Distinct Disease Profiles in Tuberculosis.
    Authors: Andrade B, Pavan Kumar N, Amaral E, Riteau N, Mayer-Barber K, Tosh K, Maier N, Conceicao E, Kubler A, Sridhar R, Banurekha V, Jawahar M, Barbosa T, Manganiello V, Moss J, Fontana J, Marciano B, Sampaio E, Olivier K, Holland S, Jackson S, Moayeri M, Leppla S, Sereti I, Barber D, Nutman T, Babu S, Sher A
    J Immunol, 2015-08-12;195(6):2763-73.
    Species: Human
    Sample Types: Plasma
  7. Overexpression of MMP-9 and its inhibitors in blood mononuclear cells after myocardial infarction--is it associated with depressive symptomatology?
    Authors: Jonsson S, Lundberg A, Jonasson L
    PLoS ONE, 2014-08-25;9(8):e105572.
    Species: Human
    Sample Types: Cell Culture Supernates

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