TopBP1 (DNA topoisomerase 2-binding protein 1) is a 175-180 kDa member of a critical DNA replication repair complex. It is widely expressed, and appears to serve as an intermediary in DNA replication. When a replication fork is stalled during S phase, DNA continues to unwind ahead of the stall. This becomes associated with RPA, which is essential for ATR and subsequent Chk1 activation. TopBP1 is key to ATR activation, and appears to first bind to DNA:pol-alpha, followed by a 9-1-1 complex and Rad17 interaction that leads to ATR stimulation. Human TopBP1 is 1522 amino acids (aa) in length. It contains eight BRCT domains (aa 101-1486) that interact with distinct binding partners. BRCT1 and 2 binds to Rad9 (aa 101-294), while BRCT 6 and 7 are involved in ATR activation. There are 12 utilized phosphorylation sites and two nuclear localization signals. Three isoform variants have been described. One contains an alternative start site at Met88, a second shows an 18 aa substitution for aa 1198-1522, and a third possesses a six aa substitution for aa 1292-1522. Over aa 88-194, human TopBP1 shares 88% aa identity with mouse TopBP1.
Human TopBP1 Alexa Fluor® 405‑conjugated Antibody
R&D Systems | Catalog # AF7120V
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Met88-Asp194
Accession # Q92547
Specificity
Clonality
Host
Isotype
Applications for Human TopBP1 Alexa Fluor® 405‑conjugated Antibody
Immunohistochemistry
Formulation, Preparation, and Storage
Purification
Formulation
Shipping
Stability & Storage
Background: TopBP1
Long Name
Alternate Names
Gene Symbol
UniProt
Additional TopBP1 Products
Product Documents for Human TopBP1 Alexa Fluor® 405‑conjugated Antibody
Certificate of Analysis
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Product Specific Notices for Human TopBP1 Alexa Fluor® 405‑conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars