Human TRF-1 Antibody Summary
Accession # P54274
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human TRF‑1 by Western Blot. Western blot shows lysates of Jurkat human leukemic T cell line and Raji human Burkitt's lymphoma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Sheep Anti-Human TRF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5300) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for TRF-1 was detected at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
TRF-1 (telomeric repeat-binding factor 1; also Pin2) is a nuclear protein that participates in telomere homeostasis. Although its predicted molecular weight is 50 kDa, it runs anomalously at 60 kDa in SDS-PAGE. TRF-1 binds as a dimer to the ends of chromosomes (telomeres), where it blocks telomerase activity, promoting normal cell senescence and turnover. Human TRF-1 is 439 amino acids (aa) in length. It contains an N-terminal acidic region (aa 2‑64), a dimerization domain (aa 65‑265), an NLS (aa 337‑356), and a DNA binding HTH myb-type domain (aa 375‑432). Phosphorylation of TRF-1 at Ser435 mediates its binding to DNA. One alternate splice form shows a deletion of aa 295‑315 that may be accompanied by a 36 aa N-terminal extension. Over aa 136‑295, human TRF-1 shares 72% aa identity with mouse TRF-1.
Citation for Human TRF-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
FANCM limits ALT activity by restricting telomeric replication stress induced by deregulated BLM and R-loops
Authors: B Silva, R Pentz, AM Figueira, R Arora, YW Lee, C Hodson, H Wischnewsk, AJ Deans, CM Azzalin
Nat Commun, 2019;10(1):2253.
Sample Types: Cell Lysates
Applications: Western Blot
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