Human TRF-1 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human TRF-1 in Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human TRF-1
    Lys136-Lys295
    Accession # P54274
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human TRF‑1 by Western Blot.

Western blot shows lysates of Jurkat human leukemic T cell line and Raji human Burkitt's lymphoma cell line. Gels were loaded with 30 μg of whole cell lysate (WCL), 20 μg of cytoplasmic (Cyto), and 10 μg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Sheep Anti-Human TRF‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5300) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for TRF‑1 was detected at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TRF-1

TRF-1 (telomeric repeat-binding factor 1; also Pin2) is a nuclear protein that participates in telomere homeostasis. Although its predicted molecular weight is 50 kDa, it runs anomalously at 60 kDa in SDS-PAGE. TRF-1 binds as a dimer to the ends of chromosomes (telomeres), where it blocks telomerase activity, promoting normal cell senescence and turnover. Human TRF-1 is 439 amino acids (aa) in length. It contains an N-terminal acidic region (aa 2‑64), a dimerization domain (aa 65‑265), an NLS (aa 337‑356), and a DNA binding HTH myb-type domain (aa 375‑432). Phosphorylation of TRF-1 at Ser435 mediates its binding to DNA. One alternate splice form shows a deletion of aa 295‑315 that may be accompanied by a 36 aa N-terminal extension. Over aa 136‑295, human TRF-1 shares 72% aa identity with mouse TRF-1.

  • Long Name:
    Telomeric Repeat Binding Factor
  • Entrez Gene IDs:
    7013 (Human); 21749 (Mouse); 297758 (Rat)
  • Alternate Names:
    NIMA-interacting protein 2; PIN-2; PIN2hTRF1-AS; Telomeric protein Pin2/TRF1; telomeric repeat binding factor (NIMA-interacting) 1; telomeric repeat binding factor 1; telomeric repeat binding protein 1; telomeric repeat-binding factor 1; TERF1; TRBF1; TRF1; TRF-1; TRF1FLJ41416; TRFt-TRF1; TTAGGG repeat-binding factor 1
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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