Cytokine Multiplex Assay Kits allow researchers to simultaneously detect the levels of multiple cytokines in a single sample. This is particularly useful for identifying the wide array of changes that can be associated with different pathological conditions. For multianalyte cytokine detection, we offer membrane-based Proteome Profiler Cytokine Antibody Arrays, bead-based Luminex® Assays, and Simple Plex automated ELISAs. For our antibody arrays, we offer human, mouse, or rat XL Cytokine Antibody Arrays that detect 105, 111, or 79 analytes respectively, including interleukins, interferons, chemokines, and members of the tumor necrosis factor superfamily. Our standard Luminex Assays for human, mouse, rat, porcine, and non-human primate (NHP) may also be used to detect many of these same analytes. With a menu of more than 490 analytes to select from, multiplexing with our Luminex assays provides the ability to simultaneously assess up to 50 analytes using magnetic microparticles. Experience consistent biomarker detection and ultra-sensitive results in less than 90 minutes with Ella, our automated benchtop ELISA. Simple Plex offers an extensive selection of over 250 fully validated assays essential to complete your cytokine research. Whether you want a stocked panel, ready to ship in just 2 days, a custom panel with up to 8 assays specific to your research, or a single assay cartridge for up to 72 samples ran simultaneously, Simple Plex has the solution to your research needs.
Human Tumor Biomarker Luminex® Performance Assay
R&D Systems | Catalog # FCSTM25
Luminex High Performance Assays
Human Tumor Biomarker Luminex® Performance Assay is specifically designed for multiplex detection of 27 human analytes in serum, plasma, or tissue culture supernatants. Luminex High Performance Assays go through rigorous development, validation, and quality control testing to ensure the best performance of your multiplex assay.
This assay is customizable, select some or all the analytes in the entire panel. Customize your panel with the Luminex Assay Customization Tool.
Key Product Details
Panel
Assay Type
Format
Species
Analytes Detected
- CA125/MUC16
- CA15-3/MUC-1
- CA19-9
- CD40 Ligand/TNFSF5
- CEACAM-5/CD66e
- CYFRA21-1
- Chorionic Gonadotropin beta Chain (HCG beta)
- Chromogranin A
- Enolase 2/Neuron-specific Enolase
- FGF basic/FGF2/bFGF
- Fas/TNFRSF6/CD95
- Fas Ligand/TNFSF6
- Glypican 1
- HE4/WFDC2
- HGF
- IL-6
- IL-8/CXCL8
- Kallikrein 3/PSA
- Leptin/OB
- Osteopontin/OPN
- Prolactin
- SCF/c-kit Ligand
- TGF-alpha
- TNF-alpha
- Thyroglobulin
- VEGF
- alpha-Fetoprotein/AFP
Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Customization Tool. We also offer Custom Luminex Services.
Build Your Assay
Make Selections Below to Configure Your Custom Luminex® Assay
Product Features
Magnetic Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples.
- Simultaneously profile up to 27 analytes of your choice in one sample
- Magnetic format allows for easier wash steps
- Undergoes similar validation testing as Quantikine® ELISA Kits
- Precise and Specific
- Requires a small sample volume (<50 µL)
- Run your assay in just 3-5 hours
- Mass-calibrated standards for consistent results with every new lot of material
- Enough reagents for one 96-well plate
- Quick turn-around time: Kits are typically shipped within 5-10 days of ordering*
*Please allow additional time for international orders.
Performance Validation
R&D Systems Luminex High Performance Assays undergo our most extensive validation testing. All Luminex High Performance Assay Panels are validated for use with serum and plasma samples. Some High Performance Assays are additionally validated for cell culture supernatants, milk, saliva, or urine, as indicated on the individual product data sheets. Luminex High Performance Assays are tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types. Recovery values for individual samples in validated sample types are also tested. Validation data for each analyte can be found in the product datasheet.
Kit Contents
- Premixed cocktail of antibody-coated Magnetic beads
- Premixed cocktail of biotinylated detection antibody
- Standard Cocktail(s)
- Bead Diluent
- Biotin Antibody Diluent
- Calibrator Diluent
- Wash Buffer
- Streptavidin-PE
- One flat-bottom 96-well Microplate
- Foil Plate Sealers (4)
- Mixing Bottle
- Standard Value Card**
**A standard curve must be generated each time an assay is run, utilizing values from the Certificate of Analysis provided with each Luminex Assay.
Precision
|
Intra-Assay Precision - is generated from the mean of the %CV’s from 40 reportable results across two different concentrations of analytes in a single serum assay. Inter-Assay Precision - is generated from the mean of the %CV’s across two different concentrations of analytes across thirty six different serum assays. |
||
| n=40 (20x2 levels) | n=36 | |
|---|---|---|
| Analyte | Intra-Assay (%CV) | Inter-Assay (%CV) |
| AFP | 7.0 | 10.3 |
| CA125/MUC16 | 6.8 | 9.9 |
| CA15-5/MUC-1 | 6.3 | 8.4 |
| CA19-9 | 4.0 | 10.7 |
| CD40 Ligand/TNFSF5 | 4.8 | 8.8 |
| CEACAM-5/CD66e | 8.0 | 9.7 |
| Chromogranin A | 3.5 | 9.0 |
| CYFRA21-1 | 5.7 | 9.7 |
| Enolase 2 | 8.6 | 10.8 |
| Fas/TNFRSF6/CD95 | 5.6 | 8.6 |
| Fas Ligand | 6.2 | 9.4 |
| FGF basic | 2.4 | 8.1 |
| Glypican-1 | 7.9 | 9.7 |
| HCG-beta | 4.8 | 7.9 |
| HE4/WFDC2 | 5.2 | 8.6 |
| HGF | 6.2 | 9.0 |
| IL-6 | 3.7 | 8.0 |
| IL-8/CXCL8 | 5.9 | 9.3 |
| Leptin/OB | 4.4 | 8.8 |
| MIF | 5.1 | 8.0 |
| Osteopontin/OPN | 5.9 | 9.2 |
| Kallikrein 3/PSA | 5.1 | 8.7 |
| Prolactin | 7.9 | 9.7 |
| SCF/c-kit Ligand | 6.1 | 9.7 |
| TGF alpha | 5.4 | 9.1 |
| Thyroglobulin | 9.0 | 10.0 |
| TNF alpha | 4.0 | 9.5 |
| VEGF | 4.9 | 8.7 |
Accuracy
|
Linearity - The data represents mean spiked or natural linearity in serum matrix samples (n=4). Spike Recovery - The data represents mean percent recovery of spiked standards ranging from low, medium, and high concentration in serum matrix samples (n=4). |
||
| Linearity | Spiked Recovery | |
|---|---|---|
| Analyte | Average % | Average % |
| AFP* | 117 | HE |
| CA125/MUC16 | 120 | 86.8 |
| CA15-3/MUC-1* | 113 | HE |
| CA19-9 | 132 | 63.9 |
| CD40 Ligand/TNFSF5* | 107 | HE |
| CEACAM-5/CD66e* | 123 | HE |
| Chromogranin A* | 111 | HE |
| CYFRA21-1 | 129 | 78.2 |
| Enolase 2 | 127 | 62.5 |
| Fas/TNFRSF6/CD95* | 106 | HE |
| Fas Ligand | 122 | 82.4 |
| FGF basic | 135 | 74.6 |
| Glypican-1 | 126 | 72.9 |
| HCG-B | 131 | 58.1 |
| HE4/WFDC2* | 114 | HE |
| HGF | 134 | 62.5 |
| IL-6 | 128 | 65.6 |
| IL-8/CXCL8* | 111 | HE |
| Leptin/OB* | 110 | HE |
| MIF* | 134 | HE |
| Osteopontin/OPN* | 105 | HE |
| Kallikrein-3/PSA | 122 | 78.9 |
| Prolactin* | 106 | HE |
| SCF/c-kit Ligand | 185 | 42.8 |
| TGF alpha | 122 | 77.6 |
| Thyroglobulin | 123 | 65.8 |
| TNF alpha | 170 | 54.2 |
| VEGF | 123 | 67.5 |
| *Natural linearity HE - Spiked recovery was not observed due to natural linearity in high endogenous samples | ||
Which Luminex Assay is Right for Me?
Assays for the Luminex platform are offered as High Performance Assays or Discovery Assays.
High Performance Assays are optimized and validated as complete panels with a focused set of analytes. You can choose from Fixed Panels, Premixed options, or Configurable User Mix formats depending on your needs.
Discovery Assays deliver maximum flexibility, enabling the creation of fully customized panels tailored to your research needs. With universal reagents validated for compatibility across all analytes in the menu, they support endless customization possibilities.
Additional Information About Luminex Assays
All R&D Systems Luminex Assays are designed for use with any Luminex instrument including MAGPIX, Luminex 200, FLEXMAP 3D, and the INTELLIFLEX.
Luminex assays use color-coded, magnetic microparticles coated with analyte-specific antibodies to enable multiplex quantitation. During the assay, standards and samples are added to the wells, where target analytes bind to the capture antibodies. A biotinylated detection antibody cocktail is then introduced, followed by a Streptavidin-PE conjugate that generates a fluorescent signal proportional to the amount of analyte present.
Each microparticle is identified by a unique signature and the signal intensity is measured using a Luminex instrument. These assays are designed to deliver reliable and reproducible results with validated antibody pairs and optimized protocols. They are well suited for applications that require sensitivity, accuracy, and confidence in your data while enabling efficient multiplexing to measure multiple targets simultaneously.
Scientific Data Images for Human Tumor Biomarker Luminex® Performance Assay
Human Tumor Biomarker Luminex Panel Standard Curves
Human Tumor Biomarker Luminex Panel Serum Sample Values
Serum samples from 20 apparently healthy individuals were run on the Human Tumor Biomarker Luminex Panel and sample values plotted. Minimum and maximum values shown by exterior lines, the box represents the 1st and 3rd quartiles with the center line representing the median.
Human Tumor Biomarker Luminex Panel EDTA Plasma Sample Values
Human Tumor Biomarker Luminex Panel Heparin Plasma Sample Values
Human Tumor Biomarker Luminex Panel Cell Culture Supernate Sample Values
Human Tumor Biomarker Luminex Panel Cell Lysate Sample Values
Human Tumor Biomarker Luminex Panel Tissue Lysate Sample Values
Human Tumor Biomarker Luminex Panel Healthy Controls vs. Colorectal Cancer Serum Samples
Colorectal cancer serum samples were tested alongside healthy controls on the Human Tumor Biomarker Panel and significant differences were observed over many analytes. A selection of these are shown below.
Human Tumor Biomarker Luminex Panel Healthy Controls vs. Pancreatic Cancer Serum Samples
Pancreatic cancer serum samples were tested alongside healthy controls on the Human Tumor Biomarker Panel and significant differences were observed over many analytes. A selection of these are shown below.
Human Tumor Biomarker Luminex Panel Protein-Plus Streck Plasma Sample Values
Human Tumor Biomarker Luminex Panel Streck Plasma Sample Values
Preparation and Storage
Shipping
Stability & Storage
Background: Multiplex Cytokine Assay Kits
Additional Multiplex Cytokine Assay Kits Products
Product Documents for Human Tumor Biomarker Luminex® Performance Assay
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Tumor Biomarker Luminex® Performance Assay
Luminex® is a registered trademark of Luminex Corporation.
For research use only
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