Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry, FACS

Label

PerCP (Excitation = 482 and 564 nm, Emission = 675 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 170818
View all ULBP-1 Primary Antibodies »

Product Specifications

Immunogen

BaF3 mouse pro-B cell line transfected with human ULBP-1

Specificity

Stains human ULBP-1 cell transfectants. It does not stain cells transfected with ULBP-2 or ULBP-3.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human ULBP‑1 PerCP‑conjugated Antibody

Detection of ULBP-1 antibody in Jurkat Human Cell Line antibody by Flow Cytometry.

Detection of ULBP‑1 in Jurkat Human Cell Line by Flow Cytometry.

Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human ULBP-1 PerCP-conjugated Monoclonal Antibody (Catalog # FAB1380C, filled histogram) or isotype control antibody (Catalog # IC003C, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of ULBP‑1 in Molt-4 Human Cell Line by Flow Cytometry.

Molt-4 human myeloid leukemia cell line was stained with Mouse Anti-Human ULBP-1 PerCP-conjugated Monoclonal Antibody (Catalog # FAB1380C, filled histogram) or isotype control antibody (IC003C, open histogram). Staining was performed using our Staining Membrane-associated Proteins protocol.
Detection of Human ULBP-1 by Flow Cytometry

Detection of Human ULBP-1 by Flow Cytometry

Histograms showing sample flow cytometry profiles for the ligands studied. Bars in each plot indicate the population expressing each aNKR, Gates were set using Unstained, single stained controls, fluorescence minus one, and secondary antibody alone controls. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/1999-4915/9/10/295), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human ULBP‑1 PerCP‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Jurkat human acute T cell leukemia cell line and Molt-4 human myeloid leukemia cell line

Reviewed Applications

Read 1 review rated 5 using FAB1380C in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ULBP-1

ULBP-1 is a member of a family of cell-surface proteins that function as ligands for human NKG2D. ULBP-1 has also been described under the names RaeT1I (retinoic acid early transcript), ALCAN-beta, and NKG2DL1. The name ULBP-1 derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The gene for ULBP-1 resides in a cluster of ten related genes, six of which encode potentially functional glycoproteins. Amino acid sequence identity within this family ranges from 30-95%. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2-microglobulin. They are anchored to the membrane via a GPI-linkage. ULBP-1 and several other family members are known to bind to human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma delta T cells, and CD8+ alpha beta T cells. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. ULBP-1 is expressed on some tumor cells and has been implicated in tumor surveillance (1-8).

References

  1. Cosman, D. et al. (2001) Immunity 14:123.
  2. Kubin, M. et al. (2001) Eur. J. Immunol. 31:1428.
  3. Sutherland, C. et al. (2002) J. Immunol. 168:671.
  4. Steinle, A. et al. (2001) Immunogenetics 53:279.
  5. Sutherland, C. et al. (2001) Immunol. Rev. 181:185
  6. Pende, D. et al. (2002) Cancer Res. 62:6178.
  7. Radosavljevic, M. et al. (2002) Genomics 79:114
  8. NKG2D and its Ligands, 2002; www.RnDSystems.com.

Long Name

UL16 Binding Protein-1

Alternate Names

ALCAN-beta, MULT-1, NKG2DL, NKG2DL1, RAET1I, ULBP1

Entrez Gene IDs

80329 (Human); 77777 (Mouse)

Gene Symbol

ULBP1

Additional ULBP-1 Products

Product Documents for Human ULBP‑1 PerCP‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human ULBP‑1 PerCP‑conjugated Antibody

For research use only

Citations for Human ULBP‑1 PerCP‑conjugated Antibody

Customer Reviews for Human ULBP‑1 PerCP‑conjugated Antibody (1)

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  • Human ULBP-1 PerCP-conjugated Antibody
    Name: Stephane Hua
    Application: Flow Cytometry
    Sample Tested: Peripheral blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 07/05/2018
    Human ULBP‑1 PerCP‑conjugated Antibody FAB1380C

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