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Human ULBP-1 PE-conjugated Antibody

Catalog #: FAB1380P
13 Citations Datasheet / COA / SDS
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FAB1380P
Detection of ULBP‑1 in MOLT‑4 Human Cell Line by Flow Cytometry.
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Citations (13)
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Human ULBP-1 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Stains human ULBP-1 cell transfectants. It does not stain cells transfected with ULBP-2 or ULBP-3.
Source
Monoclonal Mouse IgG2A Clone # 170818
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human ULBP-1
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of ULBP-1 antibody in MOLT-4 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of ULBP‑1 in MOLT‑4 Human Cell Line by Flow Cytometry. MOLT-4 human acute lymphoblastic leukemia cell line was stained with Mouse Anti-Human ULBP-1 PE-conjugated Monoclonal Antibody (Catalog # FAB1380P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: ULBP-1

ULBP-1 is a member of a family of cell-surface proteins that function as ligands for human NKG2D. ULBP-1 has also been described under the names RaeT1I (retinoic acid early transcript), ALCAN-beta, and NKG2DL1. The name ULBP-1 derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The gene for ULBP-1 resides in a cluster of ten related genes, six of which encode potentially functional glycoproteins. Amino acid sequence identity within this family ranges from 30-95%. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2-microglobulin. They are anchored to the membrane via a GPI-linkage. ULBP-1 and several other family members are known to bind to human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. ULBP-1 is expressed on some tumor cells and has been implicated in tumor surveillance (1-8).

References
  1. Cosman, D. et al. (2001) Immunity 14:123.
  2. Kubin, M. et al. (2001) Eur. J. Immunol. 31:1428.
  3. Sutherland, C. et al. (2002) J. Immunol. 168:671.
  4. Steinle, A. et al. (2001) Immunogenetics 53:279.
  5. Sutherland, C. et al. (2001) Immunol. Rev. 181:185
  6. Pende, D. et al. (2002) Cancer Res. 62:6178.
  7. Radosavljevic, M. et al. (2002) Genomics 79:114
  8. NKG2D and its Ligands, 2002; www.RnDSystems.com.
Long Name
UL16 Binding Protein-1
Entrez Gene IDs
80329 (Human); 77777 (Mouse)
Alternate Names
ALCAN-beta; MULT-1; N2DL1; N2DL-1; NKG2D ligand 1; NKG2DL; NKG2DL1; RAET1I; RAET1Ialcan-beta; Retinoic acid early transcript 1I; UL16 binding protein 1; UL16-binding protein 1; ULBP1; ULBP-1

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Citations for Human ULBP-1 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Venetoclax enhances NK cell killing sensitivity of AML cells through the NKG2D/NKG2DL activation pathway
    Authors: HY Wu, KX Li, WY Pan, MQ Guo, DZ Qiu, YJ He, YH Li, YX Huang
    International immunopharmacology, 2022;104(0):108497.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Expression of NK Cell Receptor Ligands on Leukemic Cells Is Associated with the Outcome of Childhood Acute Leukemia
    Authors: MV Martínez-S, JL Fuster, JA Campillo, AM Galera, M Bermúdez-C, ME Llinares, E Ramos-Elba, JF Pascual-Gá, AM Fita, H Martínez-B, JA Galián, L Gimeno, M Muro, A Minguela
    Cancers, 2021;13(10):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Chemotherapy agents stimulate dendritic cells against human colon cancer cells through upregulation of the transporter associated with antigen processing
    Authors: YH Liang, JH Tsai, YM Cheng, KY Chan, WL Hsu, CC Lee, KH Chen, KH Yeh
    Scientific Reports, 2021;11(1):9080.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Bufalin enhances the killing efficacy of NK cells against hepatocellular carcinoma by inhibiting MICA shedding
    Authors: R Fu, F Yu, W Wu, J Liu, J Li, F Guo, L Xu, F Wang, X Cui
    International immunopharmacology, 2021;101(0):108195.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. NK Cells Can Preferentially Target Prostate Cancer Stem-like Cells via the TRAIL/DR5 Signaling Pathway
    Authors: T Seki, Y Shimizu, K Ishii, Y Takahama, K Kato, T Yano
    Biomolecules, 2021;11(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. The pharmalogical reactivation of p53 function improves breast tumor cell lysis by granzyme B and NK cells through induction of autophagy
    Authors: M Chollat-Na, T Ben Safta-, D Haferssas, G Meurice, S Chouaib, J Thiery
    Cell Death Dis, 2019;10(10):695.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. iPSC-Derived Platelets Depleted of HLA Class I Are Inert to Anti-HLA Class I and Natural Killer Cell Immunity
    Authors: D Suzuki, C Flahou, N Yoshikawa, I Stirblyte, Y Hayashi, A Sawaguchi, M Akasaka, S Nakamura, N Higashi, H Xu, T Matsumoto, K Fujio, MG Manz, A Hotta, H Takizawa, K Eto, N Sugimoto
    Stem Cell Reports, 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Control of triple-negative breast cancer using ex vivo self-enriched, costimulated NKG2D CAR T cells
    Authors: Y Han, W Xie, DG Song, DJ Powell
    J Hematol Oncol, 2018;11(1):92.
    Species: Human
    Sample Types: Whole Cells
    Applications: CAR-T (Flow Cytometry)
  9. Interleukin-15 stimulates natural killer cell-mediated killing of both human pancreatic cancer and stellate cells
    Authors: JRM Van Audena, J De Waele, E Marcq, J Van Loenho, E Lion, JMJ Van den Be, R Jesenofsky, A Masamune, G Roeyen, P Pauwels, F Lardon, M Peeters, ELJ Smits
    Oncotarget, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Histone Deacetylase Inhibitors Enhance CD4 T Cell Susceptibility to NK Cell Killing but Reduce NK Cell Function
    PLoS Pathog, 2016;12(8):e1005782.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Decitabine enhances anti-CD33 monoclonal antibody BI 836858-mediated natural killer ADCC against AML blasts
    Blood, 2016;127(23):2879-89.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Oncolytic reovirus enhances rituximab-mediated antibody-dependent cellular cytotoxicity against chronic lymphocytic leukaemia.
    Authors: Parrish C, Scott G, Migneco G, Scott K, Steele L, Ilett E, West E, Hall K, Selby P, Buchanan D, Varghese A, Cragg M, Coffey M, Hillmen P, Melcher A, Errington-Mais F
    Leukemia, 2015;29(9):1799-810.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Natural killer cell-mediated shedding of ULBP2.
    Authors: Wang, Ruipeng, Sun, Peter D
    PLoS ONE, 2014;9(3):e91133.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Isotype Controls

Mouse IgG2A PE-conjugated Antibody

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Staining Reagents

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Flow Cytometry Mouse Lyse Buffer (10X)

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Flow Cytometry Staining Buffer (1X)

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