Human ULBP-1 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1380A
Human ULBP-1 APC-conjugated Antibody in Flow Cytometry
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Human ULBP-1 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Stains human ULBP-1 cell transfectants. It does not stain cells transfected with ULBP-2 or ULBP-3.
Source
Monoclonal Mouse IgG2A Clone # 170818
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human ULBP-1
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection  of ULBP‑1 in MOLT‑4 Human Cell Line by Flow Cytometry. View Larger

Detection of ULBP‑1 in MOLT‑4 Human Cell Line by Flow Cytometry. MOLT‑4 human acute lymphoblastic leukemia cell line was stained with Mouse Anti-Human ULBP‑1 APC‑conjugated Monoclonal Antibody (Catalog # FAB1380A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ULBP-1

ULBP-1 is a member of a family of cell-surface proteins that function as ligands for human NKG2D. ULBP-1 has also been described under the names RaeT1I (Retinoic Acid Early Transcript), ALCAN-beta, and NKG2DL1. The name ULBP-1 derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 Binding Proteins (ULBP). The gene for ULBP-1 resides in a cluster of ten related genes, six of which encode potentially functional glycoproteins. Amino acid sequence identity within this family ranges from 30-95%. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2-microglobulin. They are anchored to the membrane via a GPI-linkage. ULBP-1 and several other family members are known to bind to human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. ULBP-1 is expressed on some tumor cells and has been implicated in tumor surveillance (1-8).

References
  1. Cosman, D. et al. (2001) Immunity 14:123.
  2. Kubin, M. et al. (2001) Eur. J. Immunol. 31:1428.
  3. Sutherland, C. et al. (2002) J. Immunol. 168:671.
  4. Steinle, A. et al. (2001) Immunogenetics 53:279.
  5. Sutherland, C. et al. (2001) Immunol. Rev. 181:185
  6. Pende, D. et al. (2002) Cancer Res. 62:6178.
  7. Radosavljevic, M. et al. (2002) Genomics 79:114
  8. NKG2D and its Ligands, 2002; www.RnDSystems.com.
Long Name
UL16 Binding Protein-1
Entrez Gene IDs
80329 (Human); 77777 (Mouse)
Alternate Names
ALCAN-beta; MULT-1; N2DL1; N2DL-1; NKG2D ligand 1; NKG2DL; NKG2DL1; RAET1I; RAET1Ialcan-beta; Retinoic acid early transcript 1I; UL16 binding protein 1; UL16-binding protein 1; ULBP1; ULBP-1

Product Datasheets

Citation for Human ULBP-1 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Complete Remission with Reduction of High-risk Clones following Haploidentical NK Cell Therapy against MDS and AML
    Authors: AT Björklund, M Carlsten, E Sohlberg, LL Liu, T Clancy, M Karimi, S Cooley, JS Miller, M Klimkowska, M Schaffer, E Watz, KI Wikstrom, P Blomberg, BE Wahlin, M Palma, L Hansson, P Ljungman, E Hellström-, HG Ljunggren, KJ Malmberg
    Clin. Cancer Res., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human ULBP-1 APC-conjugated Antibody
By Anonymous on 06/24/2018
Application: Flow Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Human

the ligand for NKG2D. we used this antibody to analyze the expression of ULBP1 in CD4 T Cells.