Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry, Immunocytochemistry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 170818
View all ULBP-1 Primary Antibodies »

Product Specifications

Immunogen

BaF3 mouse pro-B cell line transfected with human ULBP-1

Specificity

Stains human ULBP-1 cell transfectants. It does not stain cells transfected with ULBP-2 or ULBP-3.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human ULBP‑1 APC‑conjugated Antibody

Detection of ULBP-1 antibody in MOLT-4 Human Cell Line antibody by Flow Cytometry.

Detection of ULBP‑1 in MOLT‑4 Human Cell Line by Flow Cytometry.

MOLT-4 human acute lymphoblastic leukemia cell line was stained with Mouse Anti-Human ULBP-1 APC-conjugated Monoclonal Antibody (Catalog # FAB1380A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human ULBP‑1 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: MOLT‑4 human acute lymphoblastic leukemia cell line

Reviewed Applications

Read 1 review rated 5 using FAB1380A in the following applications:

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ULBP-1

ULBP-1 is a member of a family of cell-surface proteins that function as ligands for human NKG2D. ULBP-1 has also been described under the names RaeT1I (Retinoic Acid Early Transcript), ALCAN-beta, and NKG2DL1. The name ULBP-1 derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 Binding Proteins (ULBP). The gene for ULBP-1 resides in a cluster of ten related genes, six of which encode potentially functional glycoproteins. Amino acid sequence identity within this family ranges from 30-95%. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2-microglobulin. They are anchored to the membrane via a GPI-linkage. ULBP-1 and several other family members are known to bind to human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma delta T cells, and CD8+ alpha beta T cells. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. ULBP-1 is expressed on some tumor cells and has been implicated in tumor surveillance (1-8).

References

  1. Cosman, D. et al. (2001) Immunity 14:123.
  2. Kubin, M. et al. (2001) Eur. J. Immunol. 31:1428.
  3. Sutherland, C. et al. (2002) J. Immunol. 168:671.
  4. Steinle, A. et al. (2001) Immunogenetics 53:279.
  5. Sutherland, C. et al. (2001) Immunol. Rev. 181:185
  6. Pende, D. et al. (2002) Cancer Res. 62:6178.
  7. Radosavljevic, M. et al. (2002) Genomics 79:114
  8. NKG2D and its Ligands, 2002; www.RnDSystems.com.

Long Name

UL16 Binding Protein-1

Alternate Names

ALCAN-beta, MULT-1, NKG2DL, NKG2DL1, RAET1I, ULBP1

Entrez Gene IDs

80329 (Human); 77777 (Mouse)

Gene Symbol

ULBP1

Additional ULBP-1 Products

Product Documents for Human ULBP‑1 APC‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ULBP‑1 APC‑conjugated Antibody

For research use only

Citations for Human ULBP‑1 APC‑conjugated Antibody

Customer Reviews for Human ULBP‑1 APC‑conjugated Antibody (1)

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  • Human ULBP-1 APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Peripheral blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 06/24/2018
    the ligand for NKG2D. we used this antibody to analyze the expression of ULBP1 in CD4 T Cells.

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