Rat IgG Isotype Control

Novus Biologicals | Catalog # NBP1-71666

Novus Biologicals

Key Product Details

Species Reactivity

Rat

Applications

ELISA, Fluorophore-linked immunosorbent assay, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rat IgG
View all IgG Isotype Controls »

Product Specifications

Clonality

Polyclonal

Host

Rat

Isotype

IgG

Description

This Rat IgG immunoglobulin is useful as an isotype control for Rat IgG antibody.

Scientific Data Images for Rat IgG Isotype Control

Flow Cytometry: Rat IgG Isotype Control [NBP1-71666]

Flow Cytometry: Rat IgG Isotype Control [NBP1-71666]

Flow Cytometry: Rat IgG Isotype Control [NBP1-71666] - Image from verified customer review.
ELISA: Rat IgG Isotype Control [NBP1-71666]

ELISA: Rat IgG Isotype Control [NBP1-71666]

ELISA: Rat IgG Isotype Control [NBP1-71666] - ELISA plate was coated with serially diluted Rat IgG-UNLB. Immunoglobulin was detected with Goat Anti-Rat IgG-BIOT and Mouse Anti-Rat IgM-BIOT followed by Streptavidin-HRP and quantified.

Applications for Rat IgG Isotype Control

Application
Recommended Usage

ELISA

1:100 - 1:2000

Flow Cytometry

1:10 - 1:1000

Immunoprecipitation

1:10 - 1:500
Application Notes
IP usage was reported in (PMID: 22065344) Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined.

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Formulation

BBS, pH 8.2.

Preservative

No Preservative

Concentration

Please contact technical services for concentration.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: IgG

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 10^11 variants.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Gene Symbol

IGHG1

Additional IgG Products

Product Documents for Rat IgG Isotype Control

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Rat IgG Isotype Control

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Citations for Rat IgG Isotype Control

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Protocols

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FAQs

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