Rat IL-17/IL-17A ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP1-92705

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (EDTA, citrate, heparin) (50 uL)

Sensitivity

1.0 pg/mL

Assay Range

1.6 - 100 pg/mL
View all IL-17/IL-17A ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Rat

Specificity

Crossreactivity and interference of circulating factors of the immune system were evaluated by spiking these proteins at physiologically relevant concentrations into a rat IL-17A positive sample. There was no crossreactivity detected, notably not with rat IFN gamma, rat TNF alpha, rat IL-1 alpha, rat IL-4, rat MCP-1, rat GM-CSF.

Description

The rat IL-17A ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of rat IL-17A.

Precision

Intra-Assay Precision (Precision within an assay) CV% 8.5%

Inter-Assay Precision (Precision between assays) CV% 7.6%

Recovery for Rat IL-17/IL-17A ELISA Kit (Colorimetric)

Recovery

Please see protocol for more specific Spike Recovery details.

Scientific Data Images for Rat IL-17/IL-17A ELISA Kit (Colorimetric)

ELISA: Rat IL-17/IL-17A ELISA Kit (Colorimetric) [NBP1-92705]

ELISA: Rat IL-17/IL-17A ELISA Kit (Colorimetric) [NBP1-92705]

ELISA: IL-17/IL-17A ELISA Kit [HRP] [NBP1-92705] - Standard curve

Kit Contents for Rat IL-17/IL-17A ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to rat IL-17A
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Biotin-Conjugate anti-rat IL-17A monoclonal antibody
  • Rat IL-17A Standard lyophilized, 200 pg/ml upon reconstitution
  • Sample Diluent
  • Stop Solution (1M Phosphoric acid)
  • Streptavidin-HRP
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-17/IL-17A

IL-17A, a homodimeric cytokine of about 32 kDa, is largely produced by activated memory T lymphocytes, but stimulates innate immunity and host defense. IL-17A and IL-17F both mobilize neutrophils partly through granulopoeisis and CXC chemokine induction, as well as increased survival locally. IL-17A and IL-17F production by T lymphocytes is regulated by IL-23 independent of T cell receptor activation. The Th1 and Th2 cell classification has until recently provided the framework for understanding CD4+ T cell biology and the interplay between innate and adaptive immunity. The Th17 lineage produces interleukin 17, which is highly proinflammatory and induces severe autoimmunity. Increased levels of IL-17 have been associated with several conditions, including airway inflammation, rheumatoid arthritis, intraperitoneal abscesses and adhesions, inflammatory bowel disease, allograft rejection, psoriasis, cancer and multiple sclerosis.

Long Name

Interleukin 17

Alternate Names

CTLA-8, CTLA8, IL-17A, IL17, IL17A

Gene Symbol

IL17A

Additional IL-17/IL-17A Products

Product Documents for Rat IL-17/IL-17A ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Rat IL-17/IL-17A ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Citations for Rat IL-17/IL-17A ELISA Kit (Colorimetric)

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FAQs for Rat IL-17/IL-17A ELISA Kit (Colorimetric)

Showing  1 - 2 of 2 FAQs Showing All

  • Q: I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.

    A:

    Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.

  • Q: I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.

    A:

    Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the this link to see the available fluors. The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).

  • Q: I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.

    A:

    Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.

  • Q: I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.

    A:

    Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the this link to see the available fluors. The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).

Showing  1 - 2 of 2 FAQs Showing All
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