Indo 1AM

Catalog # Availability Size / Price Qty
Indo 1AM | CAS No. 112926-02-0 | Calcium Signaling Probes and Ca2+ Indicator Dyes
1 Image
Description: Fluorescent Ca2+ indicator

Chemical Name: 2-[4-[Bis[2-[(acetoxy)methoxy]-2-oxoethyl]amino]-3-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-1H-indole-6-carboxylic acid (acetoxy)methyl ester

Purity: ≥90%

Product Details

Biological Activity

Indo 1AM is a fluorescent Ca2+ indicator (Kd for Ca2+ = 250 nM). Cell-permeable. Displays ratiometric emission wavelength change upon Ca2+ binding. Excitation/emission λ are 355/475 nm for no Ca2+ and 355/401 nm for high Ca2+. Absorption maxima are 349 and 331 nm for free anion and Ca complex, respectively. Respective emission maxima are 485 and 410 nm. Quantum yield ~0.5. Ideal for flow cytometry.

Technical Data

Soluble to 1 mM in DMSO
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

  1. A new generation of Ca2+ indicators with greatly improved fluorescence properties.
    Grynkiewicz et al.
    J.Biol.Chem., 1985;260:3440

Product Datasheets

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