Indo 1AM

Tocris Bioscience | Catalog # 6704

Fluorescent Ca2+ indicator
Tocris Bioscience
Discontinued Product
6704 has been discontinued. View all Calcium Signaling Probes and Ca2+ Indicator Dyes products.

Key Product Details

Description

Fluorescent Ca2+ indicator

Wavelength

Excitation = 355 nm, Emission = 474 nm
View all Calcium Signaling Probes and Ca2+ Indicator Dyes »

Product Description

Indo 1AM is a fluorescent Ca2+ indicator (Kd for Ca2+ = 250 nM). Cell-permeable. Displays ratiometric emission wavelength change upon Ca2+ binding. Excitation/emission λ are 355/475 nm for no Ca2+ and 355/401 nm for high Ca2+. Absorption maxima are 349 and 331 nm for free anion and Ca complex, respectively. Respective emission maxima are 485 and 410 nm. Quantum yield ~0.5. Ideal for flow cytometry..

This product is typically prepared in DMSO.

Optical Data

Emission Color Blue
λabs 355 nm
λem 474 nm
Closest Laser line 350 nm
Cell Permeable Yes
Application Flow Cytometry, Fluorescence Microscopy

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Product Specifications for Indo 1AM

Molecular Weight

1009.91

Formula

C47H51N3O22

Storage

Store at -20°C

Purity

≥90% (HPLC)

Chemical Name

2-[4-[Bis[2-[(acetoxy)methoxy]-2-oxoethyl]amino]-3-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-1H-indole-6-carboxylic acid (acetoxy)methyl ester

CAS Number

112926-02-0

PubChem ID

123918

InChI Key

CAWBRCOBJNWRLK-UHFFFAOYSA-N

SMILES

CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)C1=C(OCCOC2=C(C=CC(=C2)C2=CC3=C(N2)C=C(C=C3)C(=O)OCOC(C)=O)N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)C=C(C)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

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Background References

References are publications that support the biological activity of the product.

Product Documents for Indo 1AM

Certificate of Analysis

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Product Specific Notices for Indo 1AM

For research use only

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