INTS1 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-509

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all INTS1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide derived from a sequence between residues 150 and 200 of human KIAA1440 using the numbering given in entry XP_291222.2 (GeneID 26173).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for INTS1 Antibody - BSA Free

Western Blot: INTS1 Antibody [NB100-509]

Western Blot: INTS1 Antibody [NB100-509]

Western Blot: INTS1 Antibody [NB100-509] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-INT1 antibodyA300-361A used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: INTS1 Antibody [NB100-509]

Immunoprecipitation: INTS1 Antibody [NB100-509]

Immunoprecipitation: INTS1 Antibody [NB100-509] - Detection of human INTS1 (INT1) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-INT1 antibody NB100-509 used for IP at 3 ug per reaction. INT1 was also immunoprecipitated by rabbit anti-INT1 antibody from Company B. For blotting immunoprecipitated INT1, NB100-509 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for INTS1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 5 ug/mg lysate

Western Blot

1:1000 - 1:2500
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: INTS1

INTS1 is a subunit of the Integrator complex, which associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A; MIM 180660) and mediates 3-prime end processing of small nuclear RNAs U1 (RNU1; MIM 180680) and U2 (RNU2; MIM 180690) (Baillat et al., 2005 [PubMed 16239144]).[supplied by OMIM]

Alternate Names

DKFZp586J0619, FLJ46624, integrator complex subunit 1, NET28, UNQ1821/PRO3434

Entrez Gene IDs

26173 (Human)

Gene Symbol

INTS1

UniProt

XP_291222.2 (Human)

Additional INTS1 Products

Product Documents for INTS1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for INTS1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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