Involucrin Antibody (rIVRN/9323)

Novus Biologicals | Catalog # NBP3-23830

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG2b Kappa Clone # rIVRN/9323
View all Involucrin Primary Antibodies »

Product Specifications

Immunogen

Recombinant human full-length Involucrin protein (IVRN)

Localization

Cytoplasm.

Marker

Squamous Cell Terminal Differentiation Marker

Specificity

It recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this monoclonal antibody reacts with a 120kDa protein. It stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig. Its epitope maps between codon 421-568 of human involucrin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Store at 2 to 8C. Non-hazardous. No MSDS required.

Scientific Data Images for Involucrin Antibody (rIVRN/9323)

Involucrin Antibody (rIVRN/9323) Immunohistochemistry-Paraffin: Involucrin Antibody (rIVRN/9323) [NBP3-23830] -

Immunohistochemistry-Paraffin: Involucrin Antibody (rIVRN/9323) [NBP3-23830] -

Immunohistochemistry-Paraffin: Involucrin Antibody (rIVRN/9323) [NBP3-23830] - Formalin-fixed, paraffin-embedded human skin stained with Involucrin Antibody (rIVRN/9323). Inset: PBS instead of primary antibody; secondary only negative control.

Applications for Involucrin Antibody (rIVRN/9323)

Application
Recommended Usage

Immunohistochemistry-Paraffin

0.1-0.2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 0.1-0.2ug/ml for 30 min at RT. Staining of formalin-fixed tissues is enhanced by digestion with trypsin or Protease XXV at 1mg/ml PBS for 5 min at 37C. Note than enzyme digestion is better than citrate buffer based epitope unmasking. Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Involucrin

Stains paraffin embedded human tissue, so will be useful for studies of changes in involucrin expression in pathological studies.

Alternate Names

involucrin

Gene Symbol

IVL

Additional Involucrin Products

Product Documents for Involucrin Antibody (rIVRN/9323)

Certificate of Analysis

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Product Specific Notices for Involucrin Antibody (rIVRN/9323)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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