Rat Irisin/FNDC5 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-67959

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

37.50 pg/mL

Assay Range

62.50 - 4000 pg/mL
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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Rat

Specificity

This kit recognizes Rat FNDC5 in samples. No significant cross-reactivity or interference between Rat FNDC5 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 4.91%

Inter-Assay Precision (Precision between assays) CV% 4.88%

Recovery for Rat Irisin/FNDC5 ELISA Kit (Colorimetric)

Recovery

87-102%

Linearity

Rat Irisin/FNDC5 ELISA Kit (Colorimetric)

Scientific Data Images for Rat Irisin/FNDC5 ELISA Kit (Colorimetric)

Rat Irisin/FNDC5 ELISA Kit (Colorimetric)

ELISA: Rat Irisin/FNDC5 ELISA Kit (Colorimetric) [NBP2-67959] -

ELISA: Rat Irisin/FNDC5 ELISA Kit (Colorimetric) [NBP2-67959] - Standard Curve Reference

Kit Contents for Rat Irisin/FNDC5 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Irisin/FNDC5

Irisin, also known as FNDC5, is a peptide hormone that is upregulated in skeletal muscle and subcutaneous adipose tissue during exercise. It induces expression of the mitochondrial proteins PPAR-gamma coactivator 1 alpha (PGC1a) and uncoupling protein-1 (UCP1). It contributes to the regulation of diabetes and obesity by inducing the transition of white adipose tissue into more metabolically active beige adipose tissue. Irisin also regulates neuronal cell differentiation, neurite outgrowth, and osteoblast differentiation.

Long Name

Fibronectin Type III Domain Containing 5

Alternate Names

FNDC5, FRCP2

Gene Symbol

FNDC5

Additional Irisin/FNDC5 Products

Product Documents for Rat Irisin/FNDC5 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Rat Irisin/FNDC5 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

Citations for Rat Irisin/FNDC5 ELISA Kit (Colorimetric)

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