JAM-A Antibody (DMC278) - Chimeric

Name: JAM-A Antibody (DMC278) - Chimeric
Brand: Novus Biologicals
SKU: NBP3-48306
Price: 489 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-48306

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG₁ Clone # DMC278

View all JAM-A Primary Antibodies »

Product Specifications

Immunogen

JAM-A recombinant protein expressed in mammalian cells (Accession# Q9Y624). The exact amino acid sequence is proprietary.

Clonality

Monoclonal

Host

Human

Isotype

IgG₁

Description

This product is shipped at ambient temperature. Store at -20C to -80C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80C (Avoid repeated freezing and thawing).

Scientific Data Images for JAM-A Antibody (DMC278) - Chimeric

Flow Cytometry: JAM-A Antibody (DMC278) - Chimeric [NBP3-48306]

JAM-A protein is highly expressed on the surface of HEK293 cell membrane. Flow cytometry analysis with Anti-JAM-A (DMC278) on HEK293 cells transfected with human JAM-A (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram), and Isotype antibody on HEK293 transfected with irrelevant protein (Orange histogram).

Flow Cytometry: JAM-A Antibody (DMC278) - Chimeric [NBP3-48306]

Flow cytometry analysis of antigen binding of anti-human JAM-A mAb(NBP3-48306). (A) NBP3-48306 does not bind to CHO-S cells that do not express JAM-A. (B) A clear peak shift of NBP3-48306 was seen compared to the control when incubated with JAM-A-expressing AGS cells, indicating strong binding of NBP3-48306 to JAM-A. Antibodies were incubated at 5 ug/mL.

Applications for JAM-A Antibody (DMC278) - Chimeric

Application

Recommended Usage

Flow Cytometry

1:100

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified from cell culture supernatant

Reconstitution

Dissolve the lyophilized antibody in sterile and low endotoxin water. The recommend volume of water is lot specific. Please refer to the instructions printed on the product vial or contact technical support.

Formulation

PBS, pH 7.4, 5-8% Trehalose

Preservative

Trehalose

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C in powder form. Store at -80C once reconstituted.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: JAM-A

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor. Multiple 5' alternatively spliced variants, encoding the same protein, have been identified but their biological validity has not been established.

Long Name

Junctional Adhesion Molecule A

Alternate Names

CD321, F11R, JAMA, PAM-1

Gene Symbol

F11R

Additional JAM-A Products

Product Documents for JAM-A Antibody (DMC278) - Chimeric

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Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for JAM-A Antibody (DMC278) - Chimeric

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.