Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Fluorophore-linked immunosorbent assay, Flow Cytometry

Label

PE (Excitation = 488 nm, Emission = 575 nm)

Antibody Source

Polyclonal Goat IgG
View all Lambda Light Chain Primary Antibodies »

Product Specifications

Immunogen

Mouse paraproteins with lambda 1 and lambda 2 light chains

Reactivity Notes

Human reactivity reported in (PMID: 31350616).

Specificity

LAMBDA LIGHT CHAIN

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Lambda Light Chain Antibody [PE]

Flow Cytometry: Mouse kappa Light Chain Antibody [PE] [NB100-64190] - Analysis of BALB/c splenocytes by multiple stainnig.
Fluorophore-linked immunosorbent assay: Lambda Light Chain Antibody [PE] [NB100-64190] - FLISA plate was coated with purified mouse IgG kappa, IgM kappa, IgA kappa, IgG alpha, IgM alpha, and IgA alpha. Immunoglobulins were detected with serially diluted Goat Anti-Mouse Kappa.

Applications for Lambda Light Chain Antibody [PE]

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

<= 0.1 ug/10^6 cells

Fluorophore-linked immunosorbent assay

<= 1 ug/ml

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.1% Sodium Azide

Concentration

0.25 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Lambda Light Chain

Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.

Alternate Names

IGLC, immunoglobulin lambda constant group

Gene Symbol

IGLC1

UniProt

P0DOX7 (Human)

Additional Lambda Light Chain Products

Product Documents for Lambda Light Chain Antibody [PE]

Certificate of Analysis

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Product Specific Notices for Lambda Light Chain Antibody [PE]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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