Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Fluorophore-linked immunosorbent assay, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

Azide and BSA Free
View all Lambda Light Chain Primary Antibodies »

Product Specifications

Immunogen

Mouse paraproteins with lambda 1 and lambda 2 light chains

Reactivity Notes

Human reactivity reported in (PMID: 31350616).

Specificity

LAMBDA LIGHT CHAIN

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Lambda Light Chain Antibody - Azide and BSA Free

Immunohistochemistry-Paraffin: Lambda Light Chain Antibody - Azide and BSA Free [NB100-62139]

Immunohistochemistry-Paraffin: Lambda Light Chain Antibody - Azide and BSA Free [NB100-62139]

Immunohistochemistry-Paraffin: Lambda Light Chain Antibody [NB100-62139] - Mouse spleen section was stained with Goat Anti-Mouse Lambda-UNLBfollowed by Donkey Anti-Goat IgG(H+L), Mouse/Rat SP ads-AP Red AP, and hematoxylin.
Flow Cytometry: Lambda Light Chain Antibody - Azide and BSA Free [NB100-62139]

Flow Cytometry: Lambda Light Chain Antibody - Azide and BSA Free [NB100-62139]

Flow Cytometry: Lambda Light Chain Antibody [NB100-62139] - Analysis using the TRITC conjugate of NB100-62139. Multiple staining of peripheral blood lymphocytes.
Flow Cytometry: Lambda Light Chain Antibody - Azide and BSA Free [NB100-62139]

Flow Cytometry: Lambda Light Chain Antibody - Azide and BSA Free [NB100-62139]

Flow Cytometry: Lambda Light Chain Antibody [NB100-62139] - Analysis of BALB/c splenocytes by multiple stainnig.
Flow Cytometry: Lambda Light Chain Antibody - Azide and BSA Free [NB100-62139]

Flow Cytometry: Lambda Light Chain Antibody - Azide and BSA Free [NB100-62139]

Flow Cytometry: Lambda Light Chain Antibody [NB100-62139] - Analysis using the Biotin conjugate of NB100-62139. Staining of Human peripheral blood lymphocytes with goat anti-Human Lambda-PE. and goat anti-Human Kappa-FITC
Flow Cytometry: Lambda Light Chain Antibody - Azide and BSA Free [NB100-62139]

Flow Cytometry: Lambda Light Chain Antibody - Azide and BSA Free [NB100-62139]

Flow Cytometry: Lambda Light Chain Antibody [NB100-62139] - Analysis using the PE conjugate of NB100-62139. Multiple staining of BALB/c splenocytes.
Fluorophore-linked immunosorbent assay: Lambda Light Chain Antibody - Azide and BSA Free [NB100-62139]

Fluorophore-linked immunosorbent assay: Lambda Light Chain Antibody - Azide and BSA Free [NB100-62139]

Fluorophore-linked immunosorbent assay: Lambda Light Chain Antibody [NB100-62139] - FLISA plate was coated with purified mouse IgG kappa, IgM kappa, IgA kappa, IgG alpha, IgM alpha, and IgA alpha. Immunoglobulins were detected with serially diluted Goat Anti-Mouse Kappa. Image using the PE format of this antibody.
Fluorophore-linked immunosorbent assay: Lambda Light Chain Antibody - Azide and BSA Free [NB100-62139]

Fluorophore-linked immunosorbent assay: Lambda Light Chain Antibody - Azide and BSA Free [NB100-62139]

Fluorophore-linked immunosorbent assay: Lambda Light Chain Antibody [NB100-62139] - FLISA plate was coated with purified human IgGk, IgMk, IgAk, IgG alpha, IgM alpha IgA. Immunoglobulins were detected with serially diluted Goat Anti-Human Lambda-TRITC Image using the TRITC format of this antibody.

Applications for Lambda Light Chain Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Flow Cytometry

1:10 - 1:1000

Immunocytochemistry/ Immunofluorescence

1:10 - 1:2000

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100 - 1:2000

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.1M BBS (pH 8.2)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lambda Light Chain

All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30, the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins).

Alternate Names

IGLC, immunoglobulin lambda constant group

Gene Symbol

IGLC1

Additional Lambda Light Chain Products

Product Documents for Lambda Light Chain Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Lambda Light Chain Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Lambda Light Chain Antibody - Azide and BSA Free

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Protocols

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