Recombinant Rat LIF Protein

Novus Biologicals | Catalog # NBP2-35263

Novus Biologicals
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Key Product Details

Source

E. coli

Applications

SDS-PAGE
View all LIF Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 180 amino acids corresponding to LIF Source: E. coli

Uniprot ID: P17777

Amino Acid Sequence: SPLPITPVNA TCAIRHPCHG NLMNQIKSQL AQLNGSANAL FISYYTAQGE PFPNNVDKLC APNMTDFPPF HANGTEKTKL VELYRMVTYL GASLTNITWD QKNLNPTAVS LQIKLNATTD VMRGLLSSVL CRLCNKYHVG HVDVPCVPDN SSKEAFQRKK LGCQLLGTYK QVISVLAQAF

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of LIF as determined by LAL method.

Predicted Molecular Mass

19.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Test in process.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Rat LIF Protein

SDS-PAGE: Recombinant Rat LIF Protein [NBP2-35263]

SDS-PAGE: Recombinant Rat LIF Protein [NBP2-35263]

SDS-Page: Rat LIF Protein [NBP2-35263]

Formulation, Preparation, and Storage

NBP2-35263
Preparation Method Lyophilized from a 0.2 um filtered concentrated solution in 1 x PBS, pH 7.4.
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 1 x PBS, pH 7.4.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LIF

Leukemia inhibitory factor (LIF) is a member of Interleukin 6 family. This protein is mainly expressed in the trophectoderm of the developing embryo, with its receptor LIFR expressed throughout the inner cell mass. LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. LIF is used in mouse embryonic stem cell culture, because that removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. It is also used in phase II clinical trial, which can assist embryo implantation in women who have failed to become pregnant despite assisted reproductive technologies (ART). Mature rat LIF shares 91 % and 82 % a.a. sequence identity with murine and human LIF, respectively.

Long Name

Leukemia Inhibitory Factor

Alternate Names

D Factor, Emfilermin, HILDA, MLPLI

Gene Symbol

LIF

Additional LIF Products

Product Documents for Recombinant Rat LIF Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Rat LIF Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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