LIME Antibody

Novus Biologicals | Catalog # NB100-1436

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all LIME Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-ENVYESIKEMGL corresponding to C-Terminus according to Uniprot Mouse NP_076173.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for LIME Antibody

Western Blot: LIME Antibody [NB100-1436]

Western Blot: LIME Antibody [NB100-1436]

Western Blot: LIME Antibody [NB100-1436] - (1ug/ml) of Mouse Heart lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Applications for LIME Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:128000

Western Blot

0.5 - 2 ug/ml
Application Notes
WB: Approx. 32 kDa band observed in mouse heart lysates (predicted MW of 31 kDa band according to NP_076173.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LIME

LIME (Lck-interacting molecule) is a new raft-associated adaptor protein involved in regulation of T cell activation by coreceptors. LIME becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors. Phospho-LIME associates with the Src family kinase Lck and its negative regulator, Csk. Ectopic expression of LIME in Jurkat T cells results in an increase of Csk in lipid rafts, increased phosphorylation of Lck and higher Ca2+ response to CD3 stimulation.

Alternate Names

dJ583P15.4, FLJ20406, Lck interacting transmembrane adaptor 1, Lck-interacting membrane protein, Lck-interacting molecule, lck-interacting transmembrane adapter 1, LIMELP8067

Entrez Gene IDs

54923 (Human); 72699 (Mouse); 362289 (Rat)

Gene Symbol

LIME1

UniProt

NP_076173.1 (Mouse)

Additional LIME Products

Product Documents for LIME Antibody

Certificate of Analysis

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Product Specific Notices for LIME Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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