Cytokine Multiplex Assay Kits allow researchers to simultaneously detect the levels of multiple cytokines in a single sample. This is particularly useful for identifying the wide array of changes that can be associated with different pathological conditions. For multianalyte cytokine detection, we offer membrane-based Proteome Profiler Cytokine Antibody Arrays, bead-based Luminex® Assays, and Simple Plex automated ELISAs. For our antibody arrays, we offer human, mouse, or rat XL Cytokine Antibody Arrays that detect 105, 111, or 79 analytes respectively, including interleukins, interferons, chemokines, and members of the tumor necrosis factor superfamily. Our standard Luminex Assays for human, mouse, rat, porcine, and non-human primate (NHP) may also be used to detect many of these same analytes. With a menu of more than 490 analytes to select from, multiplexing with our Luminex assays provides the ability to simultaneously assess up to 50 analytes using magnetic microparticles. Experience consistent biomarker detection and ultra-sensitive results in less than 90 minutes with Ella, our automated benchtop ELISA. Simple Plex offers an extensive selection of over 250 fully validated assays essential to complete your cytokine research. Whether you want a stocked panel, ready to ship in just 2 days, a custom panel with up to 8 assays specific to your research, or a single assay cartridge for up to 72 samples ran simultaneously, Simple Plex has the solution to your research needs.
Human XL Cytokine Luminex® Performance Assay 44-plex Fixed Panel
R&D Systems | Catalog # LKTM014
Key Product Details
Assay Type
Format
Species
Analytes Detected
Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Customization Tool. We also offer Custom Luminex Services.
Product Features
Magnetic Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples.
- Simultaneously profile Human Multiplex Cytokine Assay Kits with a panel of related analytes in one sample
- Magnetic format allows for easier wash steps
- Undergoes similar validation testing as Quantikine® ELISA Kits
- Precise and Specific
- Requires a small sample volume (<50 µL)
- Run your assay in just 3-5 hours
- Mass-calibrated standards for consistent results with every new lot of material
- Enough reagents for one 96-well plate
- Quick turn-around time: Kits are typically shipped within 2-3 days of ordering*
*Please allow additional time for international orders.
Performance Validation
R&D Systems Luminex High Performance Assays undergo our most extensive validation testing. All Luminex High Performance Assay Panels are validated for use with serum and plasma samples. Some High Performance Assays are additionally validated for cell culture supernatants, milk, saliva, or urine, as indicated on the individual product data sheets. Luminex High Performance Assays are tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types. Recovery values for individual samples in validated sample types are also tested. Validation data for each analyte can be found in the product datasheet.
View Luminex High Performance Human XL validation data.Base Kit Contents
- Premixed cocktail of antibody-coated Magnetic beads
- Premixed cocktail of biotinylated detection antibody
- Standard Cocktail(s)
- Bead Diluent
- Biotin Antibody Diluent
- Calibrator Diluent
- Wash Buffer
- Streptavidin-PE
- One flat-bottom 96-well Microplate
- Foil Plate Sealers (4)
- Mixing Bottle
- Standard Value Card**
- High and low controls with Control Mean Value Card
**A standard curve must be generated each time an assay is run, utilizing values from the Certificate of Analysis provided with each Luminex Assay.
Precision
|
Intra-Assay Precision - is generated from the mean of the %CV's from 40 reportable results across two different concentrations of analytes in a single serum assay Inter-Assay Precision - is generated from the mean of the %CV's across two different concentrations of analytes across 25 different serum assays. |
||
| n=40 (20x2 levels) | n=25 | |
|---|---|---|
| Analyte | Intra-Assay (%CV) | Inter-Assay (%CV) |
| B7-H1/PD-L1 | 1.8% | 11.3% |
| CCL11/Eotaxin | 3.2% | 10.0% |
| CCL19/MIP-3beta | 2.2% | 9.7% |
| CCL2/MCP-1 | 1.4% | 8.9% |
| CCL20/MIP-3 alpha | 2.4% | 9.9% |
| CCL3/MIP-1alpha | 1.5% | 7.3% |
| CCL4/MIP-1beta | 3.0% | 9.6% |
| CD40 Ligand | 3.3% | 10.6% |
| CXCL1/GRO alpha | 1.0% | 8.0% |
| CXCL10/IP-10 | 2.1% | 8.8% |
| CXCL2/GRO beta | 1.2% | 8.8% |
| CXCL8/IL-8 | 1.8% | 9.0% |
| EGF | 3.0% | 9.6% |
| FGF-basic | 4.2% | 12.6% |
| Flt-3 ligand | 2.5% | 10.1% |
| G-CSF | 1.8% | 10.2% |
| GM-CSF | 3.1% | 10.3% |
| Granzyme B | 1.8% | 11.0% |
| IFN-alpha | 2.9% | 9.9% |
| IFN-beta | 3.0% | 11.0% |
| IFN-gamma | 2.8% | 10.4% |
| IL-10 | 3.3% | 9.0% |
| IL-12 p70 | 2.3% | 10.5% |
| IL-13 | 1.6% | 9.7% |
| IL-15 | 2.4% | 9.9% |
| IL-17A | 2.5% | 11.2% |
| IL-17E/IL-25 | 1.7% | 9.2% |
| IL-1alpha | 3.0% | 10.4% |
| IL-1beta | 2.3% | 10.0% |
| IL-1ra | 2.1% | 10.3% |
| IL-2 | 2.2% | 9.2% |
| IL-3 | 2.5% | 10.1% |
| IL-33 | 2.4% | 12.3% |
| IL-4 | 3.2% | 9.7% |
| IL-5 | 2.2% | 12.0% |
| IL-6 | 2.6% | 9.8% |
| IL-7 | 2.5% | 9.8% |
| PDGF-AA | 2.8% | 15.5% |
| PDGF-AB/BB | 2.4% | 12.4% |
| RANTES | 4.7% | 17.4% |
| TGF-alpha | 3.0% | 9.8% |
| TNF-alpha | 2.2% | 8.7% |
| TRAIL | 2.0% | 10.3% |
| VEGF | 2.6% | 10.3% |
Accuracy
|
Linearity - The data represents mean spiked or natural linearity in serum matrix samples. Samples with natural linearity are marked with an astrisk. Spike Recovery - The data represents mean percent recovery of spiked standards ranging from low, medium, and high concentration in serum matrix samples. |
||||
| Linearity | Spiked Recovery | |||
|---|---|---|---|---|
| Analyte | N | Average % | N | Average % |
|
*Natural linearity |
||||
| B7-H1/PD-L1 | 4 | 106% | 4 | 109% |
| CCL11/Eotaxin | 4 | 92% | 4 | 107% |
| CCL19/MIP-3beta | 4 | 123% | 4 | 95% |
| CCL2/MCP-1 | 4* | 82% | High endogenous | |
| CCL20/MIP-3 alpha | 4 | 107% | 4 | 99% |
| CCL3/MIP-1alpha | 4 | 77% | 4 | 139% |
| CCL4/MIP-1beta | 4 | 103% | 4 | 111% |
| CD40 Ligand | 4 | 113% | 4 | 100% |
| CXCL1/GRO alpha | 4 | 80% | High endogenous | |
| CXCL10/IP-10 | 3 | 110% | High endogenous | |
| CXCL2/GRO beta | 4* | 114% | 3 | 106% |
| CXCL8/IL-8 | 4 | 118% | 4 | 96% |
| EGF | 4* | 110% | 4 | 106% |
| FGF-basic | 4 | 161% | 3 | 80% |
| Flt-3 ligand | 4 | 102% | 4 | 109% |
| G-CSF | 4 | 209% | 4 | 43% |
| GM-CSF | 4 | 96% | 4 | 110% |
| Granzyme B | 4 | 99% | 4 | 118% |
| IFN-alpha | 3 | 98% | 4 | 101% |
| IFN-beta | 4 | 148% | 4 | 69% |
| IFN-gamma | 4 | 97% | 4 | 112% |
| IL-10 | 4 | 111% | 4 | 104% |
| IL-12 p70 | 4 | 106% | 4 | 108% |
| IL-13 | 4 | 112% | 4 | 103% |
| IL-15 | 3 | 88% | 3 | 89% |
| IL-17A | 4 | 110% | 4 | 106% |
| IL-17E/IL-25 | 4 | 122% | 4 | 87% |
| IL-1alpha | 4 | 143% | 4 | 80% |
| IL-1beta | 4 | 107% | 4 | 99% |
| IL-1ra | 4* | 100% | 4 | 145% |
| IL-2 | 4 | 105% | 4 | 102% |
| IL-3 | 4 | 123% | 4 | 88% |
| IL-33 | 4 | 93% | 4 | 110% |
| IL-4 | 3 | 127% | 3 | 77% |
| IL-5 | 3 | 113% | 3 | 81% |
| IL-6 | 3 | 124% | 3 | 90% |
| IL-7 | 4 | 113% | 4 | 96% |
| PDGF-AA | 4* | 90% | High endogenous | |
| PDGF-AB/BB | 4* | 96% | High endogenous | |
| RANTES | 4* | 96% | High endogenous | |
| TGF-alpha | 4 | 117% | 4 | 96% |
| TNF-alpha | 4 | 117% | 4 | 69% |
| TRAIL | 3 | 123% | 4 | 88% |
| VEGF | 4 | 103% | 4 | 109% |
Which Luminex Assay is Right for Me?
Assays for the Luminex platform are offered as High Performance Assays or Discovery Assays.
High Performance Assays are optimized and validated as complete panels with a focused set of analytes. You can choose from Fixed Panels, Premixed options, or Configurable User Mix formats depending on your needs.
Discovery Assays deliver maximum flexibility, enabling the creation of fully customized panels tailored to your research needs. With universal reagents validated for compatibility across all analytes in the menu, they support endless customization possibilities.
Additional Information About Luminex Assays
All R&D Systems Luminex Assays are designed for use with any Luminex instrument including MAGPIX, Luminex 200, FLEXMAP 3D, and the INTELLIFLEX.
Luminex assays use color-coded, magnetic microparticles coated with analyte-specific antibodies to enable multiplex quantitation. During the assay, standards and samples are added to the wells, where target analytes bind to the capture antibodies. A biotinylated detection antibody cocktail is then introduced, followed by a Streptavidin-PE conjugate that generates a fluorescent signal proportional to the amount of analyte present.
Each microparticle is identified by a unique signature and the signal intensity is measured using a Luminex instrument. These assays are designed to deliver reliable and reproducible results with validated antibody pairs and optimized protocols. They are well suited for applications that require sensitivity, accuracy, and confidence in your data while enabling efficient multiplexing to measure multiple targets simultaneously.
Scientific Data Images for Human XL Cytokine Luminex® Performance Assay 44-plex Fixed Panel
Human XL Cytokine Luminex Panel Standard Curves
Human XL Cytokine Performance Luminex Panel Serum Sample Values
Serum samples from 20 apparently healthy individuals were run on the Human XL Cytokine Luminex High Performance Panel and sample values plotted. Minimum and maximum values shown by exterior lines, the box represents the 1st and 3rd quartiles with the center line representing the median.Preparation and Storage
Shipping
Stability & Storage
Background: Multiplex Cytokine Assay Kits
Additional Multiplex Cytokine Assay Kits Products
Product Documents for Human XL Cytokine Luminex® Performance Assay 44-plex Fixed Panel
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human XL Cytokine Luminex® Performance Assay 44-plex Fixed Panel
Luminex® is a registered trademark of Luminex Corporation.
For research use only
Citations for Human XL Cytokine Luminex® Performance Assay 44-plex Fixed Panel
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Sample Tested: Cerebrospinal FluidVerified Customer | Posted 11/24/2020The human XL Cytokine fixed panel performed as expected with low % CV's for all analytes
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