Lyophilized OneStep RT-qPCR Master Mix

Biological Activity

The Lyophilized OneStep RT-qPCR Master Mix is an optimized complete system for reproducible one-step, reverse transcription (RT) qPCR experiments using hydrolysis probes. Removal of a separate reverse transcription step reduces handling errors, and greatly reduces the time taken to obtain results. The Lyophilized OneStep RT-qPCR Master Mix is stable at ambient temperatures and can be conveniently shipped and stored at room temperature. The Lyophilized OneStep RT-qPCR Master Mix contains a thermo-stable TAQ Polymerase and MMLV as well as buffer, dNTPs, MgCl₂ and stabilizers at concentrations optimized for the enzymes. Once resuspended, only the template RNA and primer and probe mix are required to complete the experimental set up for a single tube reaction.

Lyophilized OneStep RT-qPCR Master Mix contains sufficient reagent for 150 reactions.

For further information regarding preparation, storage and use of the Lyophilized OneStep RT-qPCR Master Mix, please view the product protocol.

Technical Data

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets