MAP4K5 Antibody (3H3) - Azide and BSA Free

Novus Biologicals | Catalog # H00011183-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3H3

Format

Azide and BSA Free
View all MAP4K5 Primary Antibodies »

Product Specifications

Immunogen

MAP4K5 (AAH36013, 361 a.a. ~ 470 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SSDPNFMLQWNPFVDGANTGKSTSKRAIPPPLPPKPRISSYPEDNFPDEEKASTIKHCPDSESRAPQILRRQSSPSCGPVAETSSIGNGDGISKLMSENTEGSAQAPQLP

Specificity

MAP4K5 - mitogen-activated protein kinase kinase kinase kinase 5

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for MAP4K5 Antibody (3H3) - Azide and BSA Free

Western Blot: MAP4K5 Antibody (3H3) [H00011183-M01]

Western Blot: MAP4K5 Antibody (3H3) [H00011183-M01]

Western Blot: MAP4K5 Antibody (3H3) [H00011183-M01] - Analysis of MAP4K5 expression in transfected 293T cell line by MAP4K5 monoclonal antibody (M01), clone 3H3.Lane 1: MAP4K5 transfected lysate(95.04 KDa).Lane 2: Non-transfected lysate.
Western Blot: MAP4K5 Antibody (3H3) [H00011183-M01]

Western Blot: MAP4K5 Antibody (3H3) [H00011183-M01]

Western Blot: MAP4K5 Antibody (3H3) [H00011183-M01] - Western Blot analysis of MAP4K5 expression in 293 ( Cat # L026V1 ).
Western Blot: MAP4K5 Antibody (3H3) [H00011183-M01]

Western Blot: MAP4K5 Antibody (3H3) [H00011183-M01]

Western Blot: MAP4K5 Antibody (3H3) [H00011183-M01] - Analysis of MAP4K5 over-expressed 293 cell line, cotransfected with MAP4K5 Validated Chimera RNAi ( Cat # H00011183-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MAP4K5 monoclonal antibody (M01) clone 3H3 (Cat # H00011183-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
ELISA: MAP4K5 Antibody (3H3) [H00011183-M01]

ELISA: MAP4K5 Antibody (3H3) [H00011183-M01]

ELISA: MAP4K5 Antibody (3H3) [H00011183-M01] - Detection limit for recombinant GST tagged MAP4K5 is approximately 0.03ng/ml as a capture antibody.

Applications for MAP4K5 Antibody (3H3) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate, transfected lysate and recombinant protein for western blot. It has also been used for RNAi validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MAP4K5

This gene encodes a member of the serine/threonine protein kinase family, that is highly similar to yeast SPS1/STE20 kinase. Yeast SPS1/STE20 functions near the beginning of the MAP kinase signal cascades that is essential for yeast pheromone response. This kinase was shown to activate Jun kinase in mammalian cells, which suggested a role in stress response. Two alternatively spliced transcript variants encoding the same protein have been described for this gene.

Long Name

Hepatocyte Pogenitor Kinase-like/Germinal Center Knase-like Kinase

Alternate Names

GCKR, KHS1, MAPKKKK5

Entrez Gene IDs

11183 (Human)

Gene Symbol

MAP4K5

OMIM

604923 (Human)

UniProt

AAH36013 (Human)

Additional MAP4K5 Products

Product Documents for MAP4K5 Antibody (3H3) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MAP4K5 Antibody (3H3) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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