MBD1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-59293

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MBD1 Primary Antibodies »

Product Specifications

Immunogen

The exact immunogen is propietary information.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MBD1 Antibody - BSA Free

Western Blot: MBD1 Antibody [NBP2-59293]

Western Blot: MBD1 Antibody [NBP2-59293]

Western Blot: MBD1 Antibody [NBP2-59293] - Nuclear extracts of HeLa cells (40 ug) were analysed by Western blot using the antibody against MBD1 diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right, the marker (kDa) is shown on the left.
Chromatin Immunoprecipitation: MBD1 Antibody [NBP2-59293] - ChIP assays were performed using human osteosarcoma (U2OS) cells, the antibody against MBD1 and optimized PCR primer sets. Sheared chromatin from 1x10e6 cells and 1.5 ug of antibody were used per ChIP experiment. Beads only were used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the MLH1 gene (used as a positive control) and CDC6 gene (used as a negative control). Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
ELISA: MBD1 Antibody [NBP2-59293]

ELISA: MBD1 Antibody [NBP2-59293]

ELISA: MBD1 Antibody [NBP2-59293] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human MBD1, crude serum and Flow Through. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1:20,000.

Applications for MBD1 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1.5 ug

ELISA

1:1000

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide and 0.05% ProClin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MBD1

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from methylated promoters; in addition, variants with three CXXC domains also repress unmethylated promoter activity. MBD1 and MBD2 map very close to each other on chromosome 18q21.

Alternate Names

CXXC-type zinc finger protein 3, methyl-CpG binding domain protein 1, methyl-CpG binding domain protein 1 isoform PCM1, methyl-CpG-binding domain protein 1, Methyl-CpG-binding protein MBD1, PCM1CXXC3RFT, Protein containing methyl-CpG-binding domain 1, the regulator of fibroblast growth factor 2 (FGF-2) transcription

Gene Symbol

MBD1

Additional MBD1 Products

Product Documents for MBD1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MBD1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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