MBD1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-18674

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Format

BSA Free
View all MBD1 Primary Antibodies »

Product Specifications

Immunogen

Polyclonal antibody raised in rabbit against human MBD1 (Methyl-CpG-binding domain protein 1), using 3 KLH-conjugated synthetic peptides, two containing a sequence from the C-terminal part and one containing a sequence from the N-terminal part of the protein.

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for MBD1 Antibody - BSA Free

Western Blot: MBD1 Antibody [NBP3-18674]

Western Blot: MBD1 Antibody [NBP3-18674]

Western Blot: MBD1 Antibody [NBP3-18674] - Figure 2. Western blot analysis using the antibody directed against MBD1 HeLa cells were transfected with an expression vector for TY1-tagged MBD1. The presence of the TY1-MBD1 in the cell lysates was demonstrated by western blot analysis with the antibody against MBD1 diluted 1:1,000 in TBST containing 5% milk powder. The position of the protein of interest is indicated on the right, the marker (in kDa) is shown on the left.
ELISA: MBD1 Antibody [NBP3-18674]

ELISA: MBD1 Antibody [NBP3-18674]

ELISA: MBD1 Antibody [NBP3-18674] - Figure 1. Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human MBD1 in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:54,000.

Applications for MBD1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:1000

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide and 0.05% ProClin 300

Concentration

0.6 mg/ml

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: MBD1

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from methylated promoters; in addition, variants with three CXXC domains also repress unmethylated promoter activity. MBD1 and MBD2 map very close to each other on chromosome 18q21.

Alternate Names

CXXC-type zinc finger protein 3, methyl-CpG binding domain protein 1, methyl-CpG binding domain protein 1 isoform PCM1, methyl-CpG-binding domain protein 1, Methyl-CpG-binding protein MBD1, PCM1CXXC3RFT, Protein containing methyl-CpG-binding domain 1, the regulator of fibroblast growth factor 2 (FGF-2) transcription

Gene Symbol

MBD1

UniProt

Q9UIS9 (Human)

Additional MBD1 Products

Product Documents for MBD1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MBD1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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