Human MBL ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-60089

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and tissue homogenates (50 - 100 uL)

Sensitivity

0.171 ng/mL

Assay Range

1.563 ng/mL - 100 ng/mL
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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This assay has high sensitivity and excellent specificity for detection of human MBP/MBL. No significant cross-reactivity or interference between human MBP/MBL and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV 8

Inter-Assay Precision (Precision between assays) %CV 10

Scientific Data Images for Human MBL ELISA Kit (Colorimetric)

ELISA: Human MBL ELISA Kit (Colorimetric) [NBP2-60089]

ELISA: Human MBL ELISA Kit (Colorimetric) [NBP2-60089]

ELISA: Human MBL ELISA Kit (Colorimetric) [NBP2-60089] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Human MBL ELISA Kit (Colorimetric)

Human MBL ELISA Kit (Colorimetric) [NBP2-60089] -

Human MBL ELISA Kit (Colorimetric) [NBP2-60089] - Standard Curve Reference

Kit Contents for Human MBL ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Assay plate (12 x 8 coated Microwells)
  • Biotin-antibody (100 x concentrate)
  • Biotin-antibody Diluent
  • HRP-avidin (100 x concentrate)
  • HRP-avidin Diluent
  • Instruction manual
  • Sample Diluent
  • Standard (Freeze dried)
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: MBL

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MBP/MBL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MBP/MBL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MBP/MBL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MBP/MBL bound in the initial step. The color development is stopped and the intensity of the color is measured.

Long Name

Mannose Binding Lectin

Alternate Names

COLEC1, Collectin-1, MBL2, MBL2D, MBP1

Gene Symbol

MBL2

Additional MBL Products

Product Documents for Human MBL ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Human MBL ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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